alexa LC MS/MS Characterization of Forced Degradation Products of Tetrabenazine
ISSN: 2150-3494

Chemical Sciences Journal
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

LC MS/MS Characterization of Forced Degradation Products of Tetrabenazine

Swetha Parsha1,2*, Ravindra Kumar Y1 and Ravichander M3

1Department of Analytical Research and Development, Integrated Product Development, Dr. Reddy’s Laboratories, Hyderabad, Telangana, India

2Department of Chemistry, JNTU, Hyderabad, Telangana, India

3Department of Chemistry, MGIT, Hyderabad, Telangana, India

 

 

 

*Corresponding Author:

 

Swetha Parsha
Department of Analytical Research and Development
Dr. Reddy’s Laboratories, Hyderabad, India
Tel: +918790765770
Fax: +914044346194
E-mail: [email protected]

Received date: December 30, 2015; Accepted date: December 31, 2015; Published date: January 08, 2016

Citation: Swetha P, Kumar YR, Ravichander M (2016) LC–MS/MS Characterization of Forced Degradation Products of Tetrabenazine. Chem Sci J 7:116. doi:10.4172/2150-3494.1000116

Copyright: © 2016 Swetha P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A rapid, precise and reliable LC-MS/MS method has been developed for the identification and characterization of stressed degradation products of Tetrabenazine. Tetrabenazine is a drug, for the symptomatic treatment of hyperkinetic movement disorder. Tetrabenazine works mainly as a VMAT inhibitor. It promotes the early metabolic degradation of monoamines, in particular the neurotransmitter dopamine. Tetrabenazine was subjected to hydrolysis acidic, alkaline, neutral peroxidation, light and thermal stress conditions as per ICH-specified conditions. The drug showed degradation under peroxidation, thermal, acid and base hydrolysis stress conditions. However, it was stable to neutral stress conditions and light degradation. A total of 4 degradation products were observed and the chromatographic separation of the drug and its degradation products were achieved on Inertsil ODS-3V 150 mm × 4.6 mm, i.d., 5 μm column using 0.01 M ammonium acetate and ACN in the ratio of 640:360 as mobile phase-A and 900:100 ratio of ACN:Water as mobile phase –B. The degradation products were characterized by LC–MS/MS and its fragmentation pathways were proposed. Probable possible structures were drawn based on parent and daughter molecular ions.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords