alexa LC/MS Based Monitoring of Endogenous Decay Markers for
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

LC/MS Based Monitoring of Endogenous Decay Markers for Quality Assessment of Serum Specimens

Jörg Oliver Thumfart1#, Nada Abidi3#, Sonani Mindt1, Victor Costina1, Ralf Hofheinz2, Frank Klawonn3,4, Michael Neumaier1 and Peter Findeisen1*

1Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany

2III. Medical Clinic, Medical Faculty Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany

3Department of Computer Science, Ostfalia University of Applied Sciences, Saldahlumer Str.46/48, D-38302 Wolfenbuettel, Germany

4Biostatistics, Helmholtz Centre for Infection Research, Inhoffenstraße 7, D-38124 Braunschweig, Germany

#Both authors contributed equally to this work

*Corresponding Author:
Peter Findeisen
MD, Institute for Clinical Chemistry
Medical Faculty Mannheim of the University of Heidelberg
Theodor-Kutzer-Ufer 1, 68167 Mannheim, Germany
Tel: +49-621 383 3483
Fax: +49-621 383 3819
E-mail: peter.findeisen@medma.uni-heidelberg.de

Received date: February 26, 2015; Accepted date: April 30, 2015; Published date: May 04, 2015

Citation: Thumfart JO, Abidi N, Mindt S, Costina V, Hofheinz R, et al. (2015) LC/ MS Based Monitoring of Endogenous Decay Markers for Quality Assessment of Serum Specimens. J Proteomics Bioinform 8:091-097. doi:10.4172/jpb.1000356

Copyright: © 2015 Thumfart JO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Introduction: Preanalytical variations have major impact on most biological assays. Specifically MS-based multiparametric proteomics analyses of blood specimens are seriously affected by limited protein stability due to high intrinsic proteolytic activity of serum and plasma. However, the direct analysis of sample quality (DASQ) for serum specimens is not readily available. Here we propose the mass spectrometry based monitoring of peptide patterns that are ex vivo changing in a time dependent manner to alleviate these constrains.

Materials and methods: Serum specimens from healthy controls (n=3) and patients with colorectal cancer were analyzed for a set of endogenous peptides (n=62). The respective proteolytic fragments were monitored with LC/MS at different preanalytical points in time ranging from 1 h to 48 h after blood withdrawal. An algorithm was constructed with a training set of serum specimens from colorectal cancer patients (n=30). An independent test set of patients (n=20) was used for further validation.

Results: The coefficient of determination (R2) for the linear regression of true and estimated points in time was 0.89. However, the classification accuracy for specimens with a preanalytical time span below 8 h was higher when compared to older specimens (>8 h).

Conclusion: Endogenous peptides are processed in blood specimens in a time dependent manner. This ‘proteomic degradation clock’ can be used to estimate the preanalytical quality of serum specimens. This is specifically relevant prior to in depth proteomic profiling approaches or other laborious analyses in research or diagnostic applications. Accordingly, specimens with low quality can be identified and subsequently be excluded from further analyses to avoid any unwanted preanalytical bias.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords