alexa Leaf Rust Resistance and Molecular Identification of Lr
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Leaf Rust Resistance and Molecular Identification of Lr 34 Gene in Egyptian Wheat

Fahmi AI1*, El-Shehawi AM2,3 and El-Orabey WM4

1Department of Genetics, Faculty of Agriculture, Menoufiya University, Shibin El-Kom, Egypt

2Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia

3Department of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt

4Wheat Diseases Res. Department, Plant Pathology Res. Institute, ARC, Giza, Egypt

*Corresponding Author:
Fahmi AI
Department of Genetics, Faculty of Agriculture
Menoufiya University, Shibin El-Kom, Egypt
Tel: 20 48 222 170
Fax: 0020225769495
E-mail: [email protected]

Received Date: September 19, 2015; Accepted Date: October 17, 2015; Published Date: October 24, 2015

Citation: Fahmi AI, El-Shehawi AM, El-Orabey WM (2015) Leaf Rust Resistance and Molecular Identification of Lr 34 Gene in Egyptian Wheat. J Microb Biochem Technol 7:338-343. doi:10.4172/1948-5948.1000236

Copyright: © 2015 Fahmi AI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Twenty six wheat genotypes were evaluated for their levels of adult plant resistance to leaf rust at two different locations i.e. Shibin El-Kom and Itay El-Baroud during two successive growing seasons, i.e., 2011/12 and 2012/13. The wheat varieties Sids 12, Sids 13, Misr 1 and Misr 2, Shandweel 1, Beni Sweif 4 and Beni Sweif 5 have race-specific resistance. While, the wheat genotypes Lr 34, Giza 165, Giza 168, Sakha 8, Sakha 94, Sakha 95, Gemmeiza 5, Gemmeiza 7, Gemmeiza 9, Gemmeiza 10, Gemmeiza 11 and Sohag 3 showed high levels of slow rusting resistance. On the other hand, the other tested varieties were highly susceptible or fast rusting ones i.e. Giza 160, Giza 163, Giza 164, Sakha 69, Sakha 93, Sids 1 and Giza 139. These varieties showed higher values of final rust severity (%) and AUDPC compared to the other varieties under the same field conditions. SSR marker i.e. cslv34b allele (150 bp) for Lr 34 was used to confirm the presence of the adult plant resistance gene Lr 34 in the tested genotypes.

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