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Leptin Inhibits Preproinsulin mRNA Expression Induced by Suppression of Cytokine Signalling 3 in Beta-Cells | OMICS International | Abstract
ISSN: 2167-0943

Journal of Metabolic Syndrome
Open Access

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Research Article

Leptin Inhibits Preproinsulin mRNA Expression Induced by Suppression of Cytokine Signalling 3 in Beta-Cells

Jiaqiang Zhou1*, Jiahua Wu1, Fengqin Dong2, Zhe Zhang2, Fang Wu1 and Hong Li1
1 Department of Endocrinology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China
2 Department of Endocrinology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China
Corresponding Author : Jiaqiang Zhou
Department of Endocrinology
Sir Run Run Shaw Hospital, Zhejiang University
School of Medicine, 3 East Qingchun Road
Hangzhou, 310016, Zhejiang, China
Tel: 057186006640
E-mail: [email protected]
Received March 02, 2014; Accepted April 24, 2014; Published April 26, 2014
Citation: Zhou J, Wu J, Dong F, Zhang Z, Wu F, et al. (2014) Leptin Inhibits Preproinsulin mRNA Expression Induced by Suppression of Cytokine Signalling 3 in Beta-Cells. J Metabolic Synd 3:153. doi:10.4172/2167-0943.1000153
Copyright: © 2014 Zhou J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Aim: To study the role of crosstalk between SOCS3 and leptin on insulin expression in rat insulinoma (RIN-5AH) cells that inducibly express SOCS3 mRNA.

Materials and Methods: SOCS3 and preproinsulin mRNA expression induced by 5 μM ponasterone A, and the effects of leptin on SOCS3 and preproinsulin mRNA levels were detected by RT-PCR and quantitative PCR, respectively. The effects of SOCS3 on STAT3 phosphorylation were investigated by Western blot analysis.

Results: We discovered that SOCS3 regulates preproinsulin mRNA levels in a dose-dependent and timedependent manner. The insulin-suppressing effect of leptin appears to be mediated through reducing the suppressive effects of SOCS3 on STAT3 phosphorylation.

Conclusion: Our findings suggest that leptin inhibits preproinsulin mRNA expression induced by SOCS3 in RIN-5AH beta-cells.

Keywords

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