Leptin Inhibits Preproinsulin mRNA Expression Induced by Suppression of Cytokine Signalling 3 in Beta-Cells
|Jiaqiang Zhou1*, Jiahua Wu1, Fengqin Dong2, Zhe Zhang2, Fang Wu1 and Hong Li1|
|1 Department of Endocrinology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China|
|2 Department of Endocrinology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China|
|Corresponding Author :||Jiaqiang Zhou
Department of Endocrinology
Sir Run Run Shaw Hospital, Zhejiang University
School of Medicine, 3 East Qingchun Road
Hangzhou, 310016, Zhejiang, China
E-mail: [email protected]
|Received March 02, 2014; Accepted April 24, 2014; Published April 26, 2014|
|Citation: Zhou J, Wu J, Dong F, Zhang Z, Wu F, et al. (2014) Leptin Inhibits Preproinsulin mRNA Expression Induced by Suppression of Cytokine Signalling 3 in Beta-Cells. J Metabolic Synd 3:153. doi:10.4172/2167-0943.1000153|
|Copyright: © 2014 Zhou J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Aim: To study the role of crosstalk between SOCS3 and leptin on insulin expression in rat insulinoma (RIN-5AH) cells that inducibly express SOCS3 mRNA.
Materials and Methods: SOCS3 and preproinsulin mRNA expression induced by 5 μM ponasterone A, and the effects of leptin on SOCS3 and preproinsulin mRNA levels were detected by RT-PCR and quantitative PCR, respectively. The effects of SOCS3 on STAT3 phosphorylation were investigated by Western blot analysis.
Results: We discovered that SOCS3 regulates preproinsulin mRNA levels in a dose-dependent and timedependent manner. The insulin-suppressing effect of leptin appears to be mediated through reducing the suppressive effects of SOCS3 on STAT3 phosphorylation.
Conclusion: Our findings suggest that leptin inhibits preproinsulin mRNA expression induced by SOCS3 in RIN-5AH beta-cells.