Ligninases Production and Partial Purification of Mnp from Brazilian Fungal Isolate in Submerged FermentationFerhan M1,2,3*, Leao AL2, Itamar S de Melo3, Yan N1 and Sain M1
- *Corresponding Author:
- Ferhan M
Center for Biocomposites and Biomaterials Processing
Faculty of Forestry, University of Toronto
Toronto, ON, Canada M5S 3B3
E-mail: [email protected]
Received date: September 27 2012; Accepted date: October 23, 2012; Published date: October 25, 2012
Citation: Ferhan M, Leao AL, de Melo IS, Yan N, Sain M (2012) Ligninases Production and Partial Purification of Mnp from Brazilian Fungal Isolate in Submerged Fermentation. Ferment Technol 1:106. doi: 10.4172/2167-7972.1000106
Copyright: © 2012 Ferhan M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation.The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.