Lipid and Gene Interactions during Differentiation of Human Subcutaneous Adipose Tissue Stromal Vascular CellsAnna Polus1*, Beata Kiec-Wilk2, Urszula Czech1, Anna Knapp1, Urszula Cialowicz1, Alexander Sigrüner3, Tatiana Konovalova3, Gerd Schmitz3, Maciej Malecki2 and Aldona Dembinska-Kiec1
- *Corresponding Author:
- Anna Polus
Department of Clinical Biochemistry, Medical College
Jagiellonian University Kopernika 15a, 31-501 Krakow, Poland
Tel: +48 12 421 40 06
Fax: + 48 12 421 40 73
E-mail: [email protected]
Received date: June 22, 2012; Accepted date: July 24 2012; Published date: July 26, 2012
Citation: Polus A, Kiec-Wilk B, Czech U, Knapp A, Cialowicz U, et al. (2012) Lipid and Gene Interactions during Differentiation of Human Subcutaneous Adipose Tissue Stromal Vascular Cells. J Cell Sci Ther 3:132 doi: 10.4172/2157-7013.1000132
Copyright: © 2012 Polus A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Examination of the changes in cellular lipid composition, allows for an understanding in their contribution to adipocyte differentiation in health and disease. In the future the analysis of body lipid composition could offer an additional tool to diagnosing disease with the predictive capacity for prognosis.
Exposition of cells to excess of energy substrates causes activation of evolutionarily conserved adaptive mechanisms in endoplasmic reticulum stress. In preadipocytes, metabolic stress, through the activation of endoplasmic reticulum stress connected with modification of lipid composition and lipid droplet formation leads to differentiation.
The aim of the study was to examine changes in cellular lipid composition in parallel with gene expression and enzyme activity during preadipocyte differentiation using “omics” results.
Differentiation condition lead to activation of lipid droplets related protein expression and lipid droplet formation in preadipocytes. The predominant increase of the phospholipids, plasmalogens and cholesterol amounts during cell differentiation was observed. This was accompanied by stimulation of de novo synthesis of saturated fatty acids incorporated into sphingolipids and with reduction in amount of arachidonic acid. Our results indicated that the changes in lipid composition of differentiating stromal vascular fraction cells changed in parallel with gene expression and matched the functional requirements.