ÃÂ±-Lipoic Acid Exerts a Primary Prevention for the Neointimal Hyperplasia in Balloon-Injured Rat Carotid Arteries
- *Corresponding Author:
- Daisuke Kurumazuka
Drug Discovery Laboratories
Sanwa Kagaku Kenkyusho Company Limited
363 Shiosaki, Hokusei-CHO, Inabe-Shi
MIE, 511-0406, Japan
Tel: +81 594 72 6221
Fax: +81 594 82 0070
E-mail: [email protected]
Received date: May 18, 2016; Accepted date: June 15, 2016; Published date: June 24, 2016
Citation: Daisuke Kurumazukaa, Hisashi Shirakawaa, Sayaka Kimuraa, Tatsuhiko Morib, Yasuo Matsumuraa, et al. (2016) α-Lipoic Acid Exerts a Primary Prevention for the Neointimal Hyperplasia in Balloon-Injured Rat Carotid Arteries. J Pharma Reports 1:118. doi:10.4172/jpr.1000118
Copyright: © 2016 Kurumazukaa D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: It has been reported that α-lipoic acid is an anti-oxidative and anti-inflammatory agent, and exhibits beneficial effects in experimental disease models such as hypertension and acute kidney injury. In the present study, we examined effects of α-lipoic acid on neointimal hyperplasia in balloon-injured carotid arteries. Methods: Male rats underwent balloon injury of the right carotid artery with a 2F Fogarty balloon catheter. α- Lipoic acid was administrated to balloon-injured rats in the following treatment schedules; (1) days -3 to 14, (2) days -3 to 3, (3) days 7 to 14 and (4) vehicle. We evaluated the neointimal formation, NADPH oxidase-dependent superoxide production, nitrotyrosine expression and p50 subunit of nuclear factor kappa B (NF-κB p50) expression in balloon-injured and uninjured carotid arteries. Results: In vehicle-treated rats, a significant neointimal formation was observed at 14 days after balloon injury in the carotid artery, and NADPH oxidase-dependent superoxide production, nitrotyrosine expression and NF-κB p50 expression in injured right carotid arteries significantly increased at 2 days after balloon injury as compared with uninjured left carotid arteries. The long-term treatment (initiated 3 days before surgery and continued for 14 days after balloon injury; days -3 to 14) with α-lipoic acid (100 mg/kg/day) markedly reduced the neointimal formation, NADPH oxidase-mediated superoxide production, nitrotyrosine and NF-κB p50 expression. The superoxide production, nitrotyroeine and NF-κB p50 expression at 2 days after balloon injury were significantly inhibited by the short-term treatment with α-lipoic acid (days -3 to 2). The short-term treatment (days -3 to 3) also could suppress the neointimal formation at 14 days after balloon injury. Conclusion: The present study suggests that the treatment with α-lipoic acid leads to a reduction of neointimal formation, probably by inhibiting enhanced superoxide production, nitrotyrosine and NF-κB p50 activation in an early phase after balloon injury.