alexa Liquid Chromatographic Analysis of Prazosin in API, Dos
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Liquid Chromatographic Analysis of Prazosin in API, Dosage Form and Serum: Application to Drug-Metal Interaction Studies

Najma Sultana1*, Muhammad Saeed Arayne2 and Shabana Naz Shah1
1Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Pakistan
2Department of Chemistry, University of Karachi, Karachi-75270, Pakistan
Corresponding Author : Najma Sultana
Research Institute of Pharmaceutical Sciences
Faculty of Pharmacy, University of Karachi, Karachi-75270, Pakistan
E-mail: [email protected]
Received July 31, 2013; Accepted August 21, 2013; Published August 24, 2013
Citation: Sultana N, Arayne MS, Shah SN (2013) Liquid Chromatographic Analysis of Prazosin in API, Dosage Form and Serum: Application to Drug-Metal Interaction Studies. J Chromatograph Separat Techniq 4:197. doi:10.4172/2157-7064.1000197
Copyright: © 2013 Sultana N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Prazosin hydrochloride is a sympatholytic alpha-adrenergic blocker used in the treatment of anxiety, hypertension, refractory pulmonary oedema and panic disorders. Rapid, efficient, cost effective and reproducible isocratic reversed phase method has been developed and validated for the determination of prazosin in active pharmaceutical ingredient, dosage formulation and human serum using mobile phase 75:25 v/v acetonitrile:water having pH 3.20 adjusted with glacial acetic acid. Mobile phase was pumped at a flow rate of 1.5 mL min−1 using gradient elution through prepacked Nuclosil, C18 (250×4.6 mm, 10 μm) column. UV detection was performed at 250 nm. Method was validated following the ICH guidelines. Calibration curve was linear in concentration range 1.0-10 μg mL−1 with correlation coefficient 0.9999, and lower limits of detection and quantitation as 3.3 and 2.2 ng mL−1 and 9.8 and 6.1 ng mL−1 in raw material and serum respectively. Recovery was found to be in the range 99-100% and precision less than 1%. Developed method was successfully applied for routine analysis of drug pharmaceutical formulations and serum and also to study the interaction of prazosin with metal essential to human body at physiological temperature (37°C).

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