alexa Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study

Gurinder Singh, Roopa S. Pai * and Sanual Mustafa

Department of Pharmaceutics, Faculty of Pharmacy, Al-Ameen College of Pharmacy, Bangalore, Karnataka, India

*Corresponding Author:
Roopa S. Pai
Professor, Faculty of Pharmacy
Department of Pharmaceutics
Al-Ameen College of Pharmacy, Bangalore 560027
Karnataka, India
Tel: 080-22234619
Fax: 080-22225834
E-mail: roopaspai@yahoo.com, gurindersingh181@gmail.com

Received date: March 28, 2014; Accepted date: April 20, 2014; Published date: April 21, 2014

Citation: Singh G, Pai RS, Sanual M (2014) Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study. J Chromatograph Separat Techniq 5:222. doi:10.4172/2157-7064.1000222

Copyright: © 2014 Singh G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A new reverse phase liquid chromatographic method for the investigation of atazanavir in rat plasma was developed after oral administration. The chromatographic separation was achieved on Phenomenex C18 column (250 mm × 4.6 mm I.D., 5 μm), under isocratic conditions using UV detection at 249 nm. The optimized mobile phase consisted of a mixture of potassium dihydrogen phosphate (pH 3.5) and acetonitrile (58: 42, v/v) at a flow rate of 1ml/min. The system was found to produce sharp and well-resolved peak for atazanavir with retention time of 5.78 min. The linear regression analysis for the calibration curves showed a good linear correlation over the concentration range 0.050–2.0 μg/ml, with determination coefficients, R2, exceeding 0.9989. The limits of detection (LOD) and quantitation (LOQ) were found to be 0.004 μg/ml and 0.012 μg/ml, respectively. The method was successfully applied for the pharmacokinetic in rats. Atazanavir concentration in plasma reached (Cmax) was 0.087 μg/ml at 3 h after oral administration of 7.2 mg/kg/rat. The AUC0-12 was 0.4812 μg/ml*h and the apparent plasma half-life (t1/2) was 7.5 h. This method was found to be suitable for examining atazanavir concentration in rats, after oral administration of atazanavir in a single dose.

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