Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study
Gurinder Singh, Roopa S. Pai * and Sanual Mustafa
Department of Pharmaceutics, Faculty of Pharmacy, Al-Ameen College of Pharmacy, Bangalore, Karnataka, India
- *Corresponding Author:
- Roopa S. Pai
Professor, Faculty of Pharmacy
Department of Pharmaceutics
Al-Ameen College of Pharmacy, Bangalore 560027
E-mail: [email protected], [email protected]
Received date: March 28, 2014; Accepted date: April 20, 2014; Published date: April 21, 2014
Citation: Singh G, Pai RS, Sanual M (2014) Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study. J Chromatograph Separat Techniq 5:222. doi:10.4172/2157-7064.1000222
Copyright: © 2014 Singh G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A new reverse phase liquid chromatographic method for the investigation of atazanavir in rat plasma was developed after oral administration. The chromatographic separation was achieved on Phenomenex C18 column (250 mm × 4.6 mm I.D., 5 μm), under isocratic conditions using UV detection at 249 nm. The optimized mobile phase consisted of a mixture of potassium dihydrogen phosphate (pH 3.5) and acetonitrile (58: 42, v/v) at a flow rate of 1ml/min. The system was found to produce sharp and well-resolved peak for atazanavir with retention time of 5.78 min. The linear regression analysis for the calibration curves showed a good linear correlation over the concentration range 0.050–2.0 μg/ml, with determination coefficients, R2, exceeding 0.9989. The limits of detection (LOD) and quantitation (LOQ) were found to be 0.004 μg/ml and 0.012 μg/ml, respectively. The method was successfully applied for the pharmacokinetic in rats. Atazanavir concentration in plasma reached (Cmax) was 0.087 μg/ml at 3 h after oral administration of 7.2 mg/kg/rat. The AUC0-12 was 0.4812 μg/ml*h and the apparent plasma half-life (t1/2) was 7.5 h. This method was found to be suitable for examining atazanavir concentration in rats, after oral administration of atazanavir in a single dose.