Liquid Chromatographic Method for Simultaneous Quantification of Alprazolam and Mebeverine in Pharmaceutical Dosage Form
Usmangani K Chhalotiya*, Nishma M Patel, Dimal A Shah, Falgun A Mehta and Kashyap K Bhatt
Department of Pharmaceutical Chemistry and Analysis, Indukaka Ipcowala College of Pharmacy, Gujarat, India
- *Corresponding Author:
- Usmangani K Chhalotiya
Department of Pharmaceutical Chemistry and Analysis
Indukaka Ipcowala College of Pharmacy
New Vallabh Vidyanagar – 388121
E-mail: [email protected]
Received Date: April 30, 2015; Accepted Date: June 02, 2015; Published Date: June 09, 2015
Citation: Chhalotiya UK, Patel NM, Shah DA, Mehta FA, Bhatt KK (2015) Liquid Chromatographic Method for Simultaneous Quantification of Alprazolam and Mebeverine in Pharmaceutical Dosage Form. Pharm Anal Acta 6:381. doi: 10.4172/2153-2435.1000381
Copyright: ©2015 Chhalotiya UK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A sensitive, rapid, accurate and reproducible Isocratic, RP-LC method was developed for determination of Alprazolam and Mebeverine in pharmaceutical dosage form. A Sunfire C18, 5 μm column with mobile phase containing methanol: buffer (0.02M KH2PO4) (70:30). The flow rate was 1.0 ml/min and effluents were monitored at 225 nm with help of PDA detector. The retention time was 6.04 min. for ALP and 3.53 min. for MEB. The linearity was in the range of 0.05-40 μg/ ml for ALP and 0.2-40 μg/ ml for MEB. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. Due to simplicity, rapidity and accuracy of method, believe that the method will be useful for routine quality control analysis.