Liquid Chromatography Tandem Mass Spectrometry for the Measurement of Global DNA Methylation and Hydroxymethylation
Jia Liu*, Luke B Hesson and Robyn L Ward
Lowy Cancer Research Centre and Prince of Wales Clinical School, University of New South Wales, Kensington, Australia
- *Corresponding Author:
- Jia Liu
Lowy Cancer Research Centre
University of NSW
NSW 2052 Australia
E-mail: [email protected]
Received Date: June 24, 2013; Accepted Date: July 10, 2013; Published Date: July 12, 2013
Citation: Liu J, Hesson LB, Ward RL (2013) Liquid Chromatography Tandem Mass Spectrometry for the Measurement of Global DNA Methylation and Hydroxymethylation. J Proteomics Bioinform S2: 005. doi: 10.4172/jpb.S2-005
Copyright: © 2013 Liu J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DNA methylation is an important epigenetic phenomenon. Global DNA methylation levels in the genome are tissue-specific and are reduced in the context of specific developmental and pathological states. Hence accurate assays to quantify global DNA methylation are required. In this review we describe different methods for global DNA methylation measurement and explain the methodology, strengths and limitations of the gold standard assay liquid chromatography tandem mass spectrometry (LC-MS/MS) for 5-methylcytosine and 5-hydroxymethylcytosine quantitation. Measurement of global DNA methylation using LC-MS/MS has helped to elucidate the effects of environmental exposures and demethylating drugs that can alter DNA methylation levels and further studies using this technique may provide insights on the prevention and/or reversal of DNA hypomethylation.