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Liquid Chromatography–Tandem Mass Spectrometry for Simultaneous Determination of Ticarcillin and Vancomycin in Presence of Degradation Products. Application to the Chemical Stability Monitoring of Ticarcillin- Vancomycin Solutions | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Liquid Chromatography–Tandem Mass Spectrometry for Simultaneous Determination of Ticarcillin and Vancomycin in Presence of Degradation Products. Application to the Chemical Stability Monitoring of Ticarcillin- Vancomycin Solutions

Belissa E1, Nino C1, Bernard M1, Henriet T1, Sadou-Yaye H2, Surget E1, Boccadifuoco G1, Yagoubi N3 and Do B1,3*

1Assistance Publique–Hôpitaux de Paris, Agence Générale des Produits et Equipements de Santé, Département de contrôle qualité et de développement analytique,France

2Assistance Publique – Hôpitaux de Paris, Groupe hospitalier Pitié-Salpétrière, Département Pharmacie, France

3Paris-SudUniversity, Faculty of Pharmacy, EA 401 Groupe Matériaux et Santé, France

*Corresponding Author:
B Do
Université Paris-Descartes
UFR de,Pharmacie, 4 Avenue de l’Observatoire, 75006 Paris
Tel: 33662306275
Fax: 33146691492
E-mail: [email protected]

Received date: September 17, 2014; Accepted date: October 28, 2014; Published date: October 31, 2014

Citation: Belissa E, Nino C, Bernard M, Henriet T, Sadou-Yaye H, et al.(2014) Liquid Chromatography–Tandem Mass Spectrometry for Simultaneous Determination of Ticarcillin and Vancomycin in Presence of Degradation Products.Application to the Chemical Stability Monitoring of Ticarcillin-Vancomycin Solutions.J Chromatogr Sep Tech 5:243. doi:10.4172/2157-7064.1000243

Copyright: © 2014 Belissa E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Acute bacterial conjunctivitis and their complications are commonly treated by 6 mg/mL ticarcillin and 50 mg/mL vancomycin eyes drops, formulated separately in hospitals. A rapid, selective and specific LC-tandem MS method has been developed and validated for simultaneous quantification of ticarcillin and vancomycinin solution and in presence of degradation products. Atlantis® C18 Column T3 (250×4.6 mm I.D., 5 μm) and gradient mobile phase composed of 44 mM trifluoracetic acid/methanol allowed for good separation. Degradation products were separated on LC and detected with mass spectrometry in full scan mode. The method was suitable forthe stability study of ticarcillin-vancomycin pH 5.2 solutionsover an 8-day period storage at 5 ± 3°C and at room temperature (25 ± 2°C). The solutions remained stable up to 5 days at 5 ± 3°C. At room temperature, degradation of ticarcillin was accelerated in presence of vancomycin, while vancomycin stability was not further affected by ticarcillin. High-resolution mass spectrometry was used for the characterization of the degradation products.

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