Long-term Existence of Donor Adipose-derived Stem Cells in the Recipient May Modulate the Vascularized Composite Tissue Allotransplantation Survival in a Rodent ModelChien-Chang Chen1,2, Shigeru Goto3, Chia-Chun Tsai1,2 and Yur-Ren Kuo1,2*
- Corresponding Author:
- Yur-Ren Kuo, MD, PhD
Department of Plastic and Reconstructive Surgery
Kaohsiung Chang Gung Memorial Hospital
123 Ta-Pei Road, Niao-Sung, Kaohsiung 83305, Taiwan
Tel: 886-7-7317123, ext. 8002
E-mail: [email protected]; [email protected]
Received Date: December 01, 2013; Accepted Date: December 18, 2013; Published Date: December 20, 2013
Citation: Chen CC, Goto S, Tsai CC, Kuo YR (2013) Long-term Existence of Donor Adipose-derived Stem Cells in the Recipient May Modulate the Vascularized Composite Tissue Allotransplantation Survival in a Rodent Model. J Stem Cell Res Ther S6:005. doi:10.4172/2157-7633.S6-005
Copyright: © 2013 Chen CC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Our previous studies have demonstrated donor adipose-derived stem cells (ASCs) combined with short-term immunosuppressant therapy could prolong vascularized composite tissue allotransplantation (VCA) survival in a rodent hind-limb model. In this study, we investigated whether the homing and migration of donor ASCs may modulate VCA survival. Orthotopic hind-limb transplants from male Wistar to Lewis rats were performed (day 0). Donor ASCs were propagated from the adipose-tissue and subculture originated from green fluorescent protein (GFP) transgenic Wistar rats. The transplanted rat (GFP-negative Lewis) received the immunosuppressive protocol similar to our previous design including short-term cyclosporine-A (CsA, days 0-+20), anti-lymphocyte serum (ALS; 0.5 ml i.p.; -4, +1), and three rounds of GFP+-ASCs (2 × 106 cells/time, i.v. on days +1, +7, and +21). The engraftment assessment of various donor and recipient tissues were performed by using immuno-fluorescent staining. Flow cytometry was performed to quantify the GFP+-ASCs. The results revealed allotransplant survival was significantly prolonged (>100 days) exclusively in ASC-ALS-CsA group which was well correlated with long-term existence of donor GFP-positive ASCs in the recipient. Flow cytometric analysis also revealed GFP+-ASCs apparent expression in the recipient peripheral blood in 2 weeks post-ASC infusion but skewing decrease post-transplantation. The immunofluorscent staining performed on tissue biopsy samples revealed the GFP positive cells existed in the alloskin and recipient skin and liver and spleen parenchymal tissues 2 weeks post-ASC injection. However, no apparent expressions of GFP+-ASCs were found in recipient tissues at 16 weeks post-transplantation except in recipient spleen parenchyma. These indicated donor ASCs long-term existed in the recipient spleen tissue and circulating blood may lead to prolong allograft survival.