Luteolin Suppresses Ultraviolet A- and B-induced Matrix Metalloproteinase 1- and 9 Expression in Human Dermal Fibroblast Cells
- *Corresponding Author:
- Monira Pervin
Graduate School of Integrated Pharmaceutical and Nutritional Sciences
University of Shizuoka, Japan
Tel: +81 54-264-5102
E-mail: [email protected]
Received date: August 31, 2016; Accepted date: October 17, 2016; Published date: October 27, 2016
Citation: Pervin M, Unno K, Nakamura Y, Imai S (2016) Luteolin Suppresses Ultraviolet A- and B-induced Matrix Metalloproteinase 1- and 9 Expression in Human Dermal Fibroblast Cells. J Nutr Food Sci 6:560. doi: 10.4172/2155- 9600.1000560
Copyright: © 2016 Pervin M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ultraviolet (UV) irradiation induces significant changes to skin connective tissues as a result of the degradation of collagen, which is a major structural component of the extracellular matrix. This process may be mediated by matrix metalloproteinases (MMPs). In this study, we examined the protective effect of a polyphenolic flavone, luteolin, on the expression of two matrix metalloproteinases, MMP-1 and MMP-9, in UVA- and UVB-irradiated human dermal fibroblast cells. Luteolin is found in many medicinal plants as well as in a large number of vegetables, fruits and a variety of spices. It has a number of biological activities including anti-cancer, anti-oxidant, anti-inflammatory, antiallergic and immunomodulatory activities. Human dermal fibroblast (HDF) cells were treated with luteolin at 1-10 μM, then irradiated with UVA at 10 J/cm2 and UVB at 200 mJ/cm2. Cells and culture supernatant were harvested 24 h after irradiation.
Our results show that luteolin at 1-10 μM dose-dependently suppressed the expression of MMP-1 and MMP-9 genes in UVA and UVB-exposed HDF cells, as measured by quantitative real-time reverse transcription–polymerase chain reaction (qRT-PCR). Luteolin was also found to reduce the production of MMP-1 protein in UVA and UVBexposed HDF cells detected by enzyme-linked immunosorbent assay (ELISA) in a dose-dependent manner. The release of MMP-9 was also reduced by luteolin in UVB-irradiated HDF cells in a dose-dependent manner.
Our results indicate that luteolin can inhibit UV-induced MMP-1 and MMP-9 expression in HDF cells. Therefore, they may be potentially useful in the prevention and treatment of skin photoaging.