Macrophage Colony-Stimulating Factor Modulates Matrix Metalloproteinase 2 and 9 Expression in Endometrial Epithelial CellsHaifaa A Mansouri1*, Nameer B Kirma2, Peter A Binkly2, Naveen K Krishnegowda2 and Rajeshwar R Tekmal2
- Corresponding Author:
- Haifaa A Mansouri
Obstetrics and Gynecology Department
King AbdulAziz University Hospital, Saudi Arabia
E-mail: [email protected]
Received Date: March 22, 2014; Accepted Date: May 28, 2014; Published Date: May 31, 2014
Citation: Mansouri HA, Kirma NB, Binkly PA, Krishnegowda NK, Tekmal RR (2014) Macrophage Colony-Stimulating Factor Modulates Matrix Metalloproteinase 2 and 9 Expression in Endometrial Epithelial Cells. Gynecol Obstet (Sunnyvale) 4:223. doi: 10.4172/2161-0932.1000223
Copyright: © 2014 Mansouri HA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: To investigate m-Colony Stimulating Factor (CSF-1) effect on the expression of Matrix Metalloproteinases (MMP) 2 &-9 by primary endometrial epithelial cells (EECs) and Endometrial Stromal Cells (ESCs).
Design: In vitro study. Main Outcome measure: the expression of MMP 2 &-9 by EECs and ESCs.
Results: MMP-2 expression and activity of primary epithelial cells significantly decreased in CSF-1 treated EECs compared to control vehicle (P Value<0.02). A non-significant decrease in MMP-9 RNA expression and activity was observed in EECs due to CSF-1 treatment. MMP-9 activity was significantly higher in ESCs than in EECs (P Value<0.004). 26122013.
Conclusion: This study suggests that CSF-1 modulates MMP-2 expression and activity in EECs and may regulate MMP-9 via transcriptional and post-transcriptional mechanisms. Future studies are needed to further address the complex regulation of MMP expression in endometriotic lesions.