alexa Macrophage Polarization and the Effect of MicroRNA-155 Administered in Water-in-Oil-in-Water Multiple Emulsion Formulations | OMICS International
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Research Article

Macrophage Polarization and the Effect of MicroRNA-155 Administered in Water-in-Oil-in-Water Multiple Emulsion Formulations

Adwait Oka, Meghna Talekar, Qijun Ouyang, Ed Luther and Mansoor Amiji*
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA 02115 USA
Corresponding Author : Mansoor Amiji
Department of Pharmaceutical Sciences
School of Pharmacy, Northeastern University
Boston, MA 02115 USA
Tel: (617) 373-3137
Fax: (617) 373-8886
E-mail: [email protected]
Received March 11, 2015; Accepted April 28, 2015; Published April 30, 2015
Citation: Oka A, Talekar M, Ouyang Q, Luther E, Amiji M (2015) Macrophage Polarization and the Effect of MicroRNA-155 Administered in Water-in-Oil-in-Water Multiple Emulsion Formulations. J Clin Cell Immunol 6:326. doi: 10.4172/2155-9899.1000326
Copyright: ©2015 Oka A et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The tumor microenvironment is composed of range of cells including macrophages that participate in sustaining tumor growth and invasion. Tumor-associated macrophages (TAM’s) polarized to the M2 or pro-tumoral phenotype are one such population of cells highly expressed in the tumor microenvironment. The expression of microRNA’s (miR’s) is often found to be dysregulated in TAM’s and hence exogenous delivery of miR provides a strategy for macrophage phenotypic modulation to attain improved anticancer activity. In this study, we assessed the use of multiple emulsions for the delivery of miR to achieve macrophage M1 repolarization. Water-in-oil-in-water (WOW) multiple emulsions (ME) were prepared using a two-step emulsification technique for encapsulation of miR-155 and cellular uptake, transfection efficiency, and repolarization capability of J774A.1 macrophages was assessed. The repolarized macrophages were co-cultured with SKOV3 ovarian cancer cells to assess the effect on macrophage morphology, motility and apoptosis of cancer cells. The ME showed enhanced uptake and expression of miR-155, which resulted in improved M1 polarization of J774A.1 cells. Co-culture studies with SKOV3 cells indicated an alteration in apoptotic profile. Holographic assessment of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with miR-155 ME treated cells showing greater cellular interaction between the two phenotypes.

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