alexa Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7 T
ISSN: 2155-9937

Journal of Molecular Imaging & Dynamics
Open Access

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Research Article

Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7 T

Andrea Gálisová1, Daniel Jirák1,2*, Tereza Krchová3, Vít Herynek1, Eva Fábryová4, Jan Kotek3 and Milan Hájek1

1MR Unit, Department of Radiodiagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

2Institute of Biophysics and Informatics, 1st Medicine Faculty, Charles University, Prague, Czech Republic

3Department of Inorganic Chemistry, Faculty of Science, Charles University, Prague, Czech Republic

4Center of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

*Corresponding Author:
Daniel Jirák
MR Unit, Department of Radiodiagnostic and Interventional Radiology
Institute for Clinical and Experimental Medicine
Prague, Czech Republic
Tel: +420 26136 2702
E-mail: [email protected]

Received date: November 25, 2015; Accepted date: January 14, 2016; Published date: January 16, 2016

Citation: Gálisová A, Jirák D, Krchová T, Herynek V, Fábryová E, et al. (2016) Magnetic Resonance Visualization of Pancreatic Islets Labeled by PARACEST Contrast Agents at 4.7 T. J Mol Imag Dynamic 6:121. doi:10.4172/2155-9937.1000121

Copyright: © 2016 Gálisová A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Introduction: Monitoring of Pancreatic Islets (PIs) after their transplantation could provide important data related to diabetes treatment; however their visualization is conditioned by the contrast agent use. The aim of this study was to label and visualize PIs labeled by the contrast agents based on Chemical Exchange Saturation Transfer (CEST) owing an advantage of contrast switching on/off and simultaneous visualization of differently treated cell population in one MR experiment.
1.2 Material and methods: Two paramagnetic chelates with europium Eu-DO3A-ae and ytterbium Yb-DO3A-ae were tested for labeling of PIs by pinocytosis and microporation. The toxicity, labeling efficacy and detection threshold were assessed for each agent. In vitro MR imaging of the labeled islets was performed on a 4.7 T scanner using a modified turbo spin-echo (RARE) sequence.
1.3 Results: The sufficient labeling efficacy was observed only by using high agent concentration compromising islet viability. The microporation procedure was not effective for islet labeling because of its invasive nature. The islets labeled by pinocytosis were visualized in a phantom by CEST imaging, however, after a long acquisition time at 4.7 T.
1.4 Conclusion: The low sensitivity of detection and impaired cellular viability of the pancreatic islets labeled by the novel CEST contrast agents represent a challenge for the further implementation of these probes as exogenous cellular labels.

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