alexa Maintenance of the E.coli dcm Methylation of the CMV Pr
ISSN: 2168-9849

Cloning & Transgenesis
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Research Article

Maintenance of the E.coli dcm Methylation of the CMV Promoter, in Contrast to Hypomethylation of the Recognition Sequence of Transcription Factor NFkB in Transfected GBM Cells

Hua D2, Hans-Olov S1, Leif GS1, Bengt W1 and Zhong-Tian X1*
1The Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC-D10, SE-221 84 Lund, Sweden
2Division of Clinical Sciences, Department of Clinical and Experimental Medicine, Faculty of Health Science, Linköping University, S-581 85 Linköping, Sweden
*Corresponding Author : Zhong-Tian Xue
The Rausing Laboratory, Division of Neurosurgery
Department of Clinical Sciences, Lund University
BMC-D10, SE-221 84 Lund, Sweden
Tel: 46 462229260
Fax: 46462224606
E-mail: [email protected]
Received February 09, 2016; Accepted March 06, 2016; Published March 11, 2016
Citation: Hua D, Hans-Olov S, Leif GS, Bengt W, Zhong-Tian X (2016) Maintenance of the E.coli dcm Methylation of the CMV Promoter, in Contrast to Hypomethylation of the Recognition Sequence of Transcription Factor NFkB in Transfected GBM Cells. Clon Transgen 5:148. doi:10.4172/2168-9849.1000148
Copyright: © 2016 Hua D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The human cytomegalovirus (CMV) immediate early promoter has been extensively used to drive target gene expression in transgenic mammalian cells. DNA methylation of the CMV promoter has been shown to be the reason for a reduced promoter activity and silencing of the target gene. We have established an in vitro model system, in which human brain cancer cells (glioblastoma multiforme, GBM) were transfected with pAdTrack-CMV-GFP plasmid, isolated from a dcm positive (dcm+) E. coli strain. We found that in two CCTGG sequences located at position from -304 to -300 nt and from -497 to -493 nt of the CMV promoter region, the internal C was methylated in all analyzed clones, i.e., the E. coli dcm methylation pattern is maintained in the CMV promoter region after its integration into the human genome. In contrast, we found that the recognition sites for the transcription factor NFkB and certain other transcription factors in the enhancer region of the CMV promoter (from -107 to -270 nt) were hypomethylated. This might explain why the CMV promoter maintained an active mode, driving the GFP expression despite the demonstrated methylation of the CMV promoter. We noticed that the CCTGG sequence is also contained in the binding sequence motif of transcription factor NFkB. Hence we have comprehensively studied transcription factors through a database searching, and the responsive elements that contain dcm methylation sequences CCW(A/T)GG. A list of transcription factors and the corresponding regulated genes are presented.

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