alexa Male Factor Infertility Outcomes Using Magnetic Activated Cell Sorting in Intra Citoplasmatic Sperm Injection Cycles
ISSN: 2167-0250

Andrology-Open Access
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Research Article

Male Factor Infertility Outcomes Using Magnetic Activated Cell Sorting in Intra Citoplasmatic Sperm Injection Cycles

Fabrizzio Horta*, Javier Crosby, Antonio Mackenna and Christian Huidobro
Unit of Reproductive Medicine, Obstetrics and Gynaecology, Clínica las Condes, Lo Fontecilla 441, Las Condes Santiago, Region Metropolitana, Chile
Corresponding Author : Fabrizzio H
MClinEmb, Andrology Laboratory and Reproductive Medicine Laboratory Unit of Reproductive Medicine
UMR, Clinica Las Condes Lo Fontecilla 441, Las Condes
7550000, Santiago de Chile, Chile
Tel: 56979689313
E-mail: [email protected]
Rec date: January 18, 2016; Acc date: March 17, 2016; Pub date: March 25, 2016
Citation: Horta F, Crosby J, Mackenna A, Huidobro C (2016) Male Factor Infertility Outcomes Using Magnetic Activated Cell Sorting in Intra Citoplasmatic Sperm Injection Cycles. Andrology (Los Angel) 5:155. doi:10.4172/2167-0250.1000155
Copyright: © 2016 Horta, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Sperm DNA fragmentation can have negative consequences in clinical outcomes of couples undergoing Assisted Reproductive Technologies (ART). Sperm separation techniques are an important step in sperm selection for ART. The Magnetic Activated Cell Sorting (MACS) is a novel method that separates sperm by density gradient and molecular filtration to remove apoptotic sperm, which is associated to DNA damage. A decrease of DNA sperm fragmentation could improve ART outcomes. The main aim of this study was to assess the effect of MACS on fertilization, embryo development, implantation, clinical pregnancy and miscarriage rates, in couples undergoing intra cytoplasmic sperm injection (ICSI). Semen samples from 284 patients were divided in two groups; study group (n63) and control group (n = 221), analyzed by embryo transfer day (Day 3: ETD3 and day 5: ETD5) and male factor patients. Density gradients followed by MACS were used as sperm preparation method in the study group, while Swim up was the method used in the control group. Similar results were obtained between both groups for all parameters: fertilization rate of 77.18% versus 75.28%; blastulation rate of 46.66% versus 48.69%; implantation rate of 40.35% versus 35.52%; clinical pregnancy rate of 61.81% versus 59.31% and miscarriage rate of 2.94% versus 7.37%. However, statistical significant differences were found for implantation rate (study group 55.0% and control group 35.43%, p = 0.0138) in day 5 embryo transfers (ETD5). MACS technology does not improve general outcomes; however, it showed better results for ETD5. Further studies are required to identify real improvements in extended embryo culture in male infertility.

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