Manipulation of Cellular Redox State was Essential for Triggering Erythroid Differentiation of K562 Cells by Mycophenolic Acid
Elahe Mirzarazi and Razieh Yazdanparast*
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
- *Corresponding Author:
- Razieh Yazdanparast
Institute of Biochemistry and Biophysics
University of Tehran, Tehran, Iran
E-mail: [email protected]
Received date: April 19, 2017; Accepted date: May 22, 2017; Published date: June 05, 2017
Citation: Mirzarazi E, Yazdanparast R (2017) Manipulation of Cellular Redox State was Essential for Triggering Erythroid Differentiation of K562 Cells by Mycophenolic Acid. J Cell Signal 2:151.
Copyright: © 2017 Mirzarazi E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Reactive Oxygen Species (ROS) play important roles in a variety of normal cellular functions and various pathological events. Uncontrolled ROS production in various types of cancer cells has been globally reported and considered as a hallmark for new drug developments. Accordingly, in this study, we planned to investigate the probable roles of oxidants on erythroid differentiation of K562 cells under the influence of Mycophenolic acid (MPA).
Based on our results, MPA attenuated the intracellular SOD activity to provide a suitable redox environment for hemoglobinization of the exposed cells, while the cellular content of glutathione and glutathione peroxidase were augmented at the initial times of MPA-induced erythroid differentiation. These variations were accompanied by the significant reduction in the cellular ROS content in the first 8 h of MPA exposure followed by an enormous increase in ROS content by the 24 h of exposure. Furthermore, while the cells were undergoing erythroid differentiation by MPA, it became evident that p27kip1, apart from its role in cell cycle arrest, was functioning as a sensor of the intracellular oxidants.
Overall, it might be concluded that MPA-guided erythroid differentiation was strongly associated with manipulation of ROS content of the chronic myelogenous leukemia cells.