alexa Marker-free Transgenic Tomato with Engineered Mannitol Accumulation Confers Tolerance to Multiple Abiotic Stresses | OMICS International
ISSN: 2168-9296

Cell & Developmental Biology
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Research Article

Marker-free Transgenic Tomato with Engineered Mannitol Accumulation Confers Tolerance to Multiple Abiotic Stresses

Brijesh Gupta and Rajam MV*
Plant Polyamine, Transgenic and RNAi Research Laboratory, Department of Genetics, University of Delhi South Campus, India
*Corresponding Author : Rajam MV
Plant Polyamine
Transgenic and RNAi Research Laboratory
Department of Genetics, University of Delhi South Campus
Benito Juarez Road, New Delhi 110021, India
Fax: 91-1124112437
E-mail: [email protected]
Received May 11, 2013; Accepted May 27, 2013; Published May 30, 2013
Citation: Gupta B, Rajam MV (2013) Marker-free Transgenic Tomato with Engineered Mannitol Accumulation Confers Tolerance to Multiple Abiotic Stresses. Cell Dev Biol 2:113. doi:10.4172/2168-9296.1000113
Copyright: © 2013 Gupta B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The co-transformation system using mixture of two Agrobacterium cultures, one harbouring bacterial mannitol-1- phosphate dehydrogenase (mtlD) as gene of interest, whereas the other harboring the neomycin phosphotransferase (nptII) marker gene for selection, was successfully used to develop selectable marker-free transgenics of tomato with engineered mannitol accumulation, to improve tolerance against multiple abiotic stresses. We have achieved high co-transformation frequency (up to 24%), and segregation frequency (up to 22-24%) for obtaining marker-free transgenics. Marker-free transgenic tomato plants resulted in mannitol accumulation, as evident by HPLC analysis, that leads to substantial protection of the vigour of the plants under multiple abiotic stress conditions, such as high salinity (up to 200 mM NaCl), drought (up to 15% PEG), and heavy-metal toxicity (up to 2 mM CdCl2). Under stress, transgenics performed well in comparison to wild type (WT), with high percentage of seed germination and seedling growth both in vitro and in vivo. They also performed better than WT, in terms of high chlorophyll content and other photosynthetic parameters such as Fv/Fm ratio, Electron Transport Rate (ETR), photosynthetic yield, and low Non Photochemical Quenching (NPQ). Thus, high mannitol level not only increases the nutritional value of the fruit, but also improves tolerance of transgenic tomato plants by increasing its physiological and photosynthetic efficiency, suggesting that carbohydrate alterations produced by mannitol biosynthesis may be linked to the stress response.

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