alexa Medicinal Components Recoverable From Sicklepod (Senna Obtusifolia) Seed: Analysis of Components by HPLC-MSn
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Medicinal Components Recoverable From Sicklepod (Senna Obtusifolia) Seed: Analysis of Components by HPLC-MSn

Harry-O’kuru RE1*, Payne-Wahl KL1 and Busman M2

1Bio-Oils Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, USA

2Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, USA

*Corresponding Author:
Harry-O’kuru RE
Bio-Oils Research Unit
National Center for Agricultural Utilization Research
Agricultural Research Service, U.S. Department of Agriculture
1815 North University Street, Peoria, Illinois 61604, USA
Tel: +1-309-681-6341
Fax: +1-309-681-668
E-mail: [email protected]

Received Date: May 02, 2012; Accepted Date: June 05, 2012; Published Date: June 07, 2012

Citation: Harry-O’kuru RE, Payne-Wahl KL, Busman M (2012) Medicinal Components Recoverable From Sicklepod (Senna obtusifolia) Seed: Analysis of Components by HPLC-MSn. J Chromatograph Separat Techniq S2:001.doi:10.4172/2157-7064.S1-001

Copyright: ©2012 Harry-O’kuru RE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Sicklepod (Senna obtusifolia L.) is synonymous both with Cassia tora L. and Cassia obtusifolia L.). It is usually viewed as a noxious weed in crop fields in southeastern United States. This plant, however, has a long history of use in traditional medicine in oriental countries. It is a prolific seed producer the constituents of which include: polysaccharides, proteins, highly colored low fat content and many phenolic compounds. The phenolic components are usually described as toxic, although recent literature shows many of these to exhibit potent therapeutic properties for human health. Pursuant to this as part of our continuing interest in the plant, we have expanded our study of the seed by extracting a mixture of anthraquinone and naphthopyrone glycosides from S. obtusifolia seed obtained from North Carolina. A survey of the composition of the extract was affected using High Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (HPLC-ESI-MS) with a variety of collision induced dissociation (CID) experiments. The major constituents of the mixture produced HPLC-ESI-MSn data consistent with: chrysophanic acid tetraglycoside; rubrufusarin and toralactone di-, tri- and tetraglycosides; torachrysone ester, di-, tri-, tetra- and pentaglycosides and cassialactone tetraglycoside. The naphthopyrone glycosides and related phenolic compounds in the seed are value-added medicinal co-products to the galactomannan polysaccharides of S. obtusifolia. FT-IR spectra of the mixture corroborate the chromatographic information obtained of the mixture of anthraquinone glycosides.

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