alexa Melanocytes from the Outer Root Sheath of Hair Follicles Cultivated on Collagen Membrane Improve their Melanotic Properties
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

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Research Article

Melanocytes from the Outer Root Sheath of Hair Follicles Cultivated on Collagen Membrane Improve their Melanotic Properties

Vuk Savkovic1*, Katharina Sülflow1, Katrin Rabe1, Marie Schneider1 and Jan-Christoph Simon2

1Translational Centre for Regenerative Medicine, Leipzig University, Germany

2Department of Dermatology, Venereology and Allergology, Leipzig University Medical Center, Leipzig, Germany

Corresponding Author:
Vuk Savkovic
Translational Centre for Regenerative Medicine
Leipzig University
Phillip-Rosenthal-Str. 55
04103 Leipzig, Germany
Tel: +49 341 9739647
Fax: +49 341 9739609
E-mail: [email protected]

Received date: November 19, 2012; Accepted date: December 19, 2012; Published date: December 21, 2012

Citation: Savkovic V, Sülflow K, Rabe K, Schneider M, Simon JC (2012) Melanocytes from the Outer Root Sheath of Hair Follicles Cultivated on Collagen Membrane Improve their Melanotic Properties. J Tissue Sci Eng S11:004. doi:10.4172/2157-7552.S11-004

Copyright: © 2012 Savkovic V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Local depigmentation disorders such as Vitiligo are detrimental for both physiological and psychological reasons and demand a causal, non-invasive treatment. The only available treatment of a kind at present is melanocyte transplantation, disadvantageously dependent on an invasive skin biopsy as melanocyte source. We have previously reported our work on an autologous, non-invasive method of differentiating melanocytes from the outer root sheath of human hair follicles for use in transplantation treatment. Choice of a suitable biocompatible, biodegradable carrier as a temporary niche for transplanted melanocytes can be crucial in such applications. Therefore, we tested decellularized bovine collagen type I scaffold membranes as a candidate for a favourable carrier of the hair follicle melanocytes.

This study provides an additional insight to the reported upgraded explant method of cultivating melanocytes from human hair follicle and the advantages of collagen I membrane as a biocompatible carrier for them. We have displayed detailed analysis and detailed interpretation of the methodological improvements, showing that the upgraded explant procedure favoured the release, migration and proliferation of cell content with more cultivating potential and manifold higher melanocyte yield. The three-dimensional culture of melanocytes, seeded on collagen I membranes, displayed regular melanocyte markers, proliferated more rapidly and produced more melanin than the two-dimensional adherent culture. We concluded that the collagen I decellularized scaffolds provided a benevolent niche to hair follicle melanocytes and even upgraded their melanotic properties, which makes collagen I membranes an excellent candidate for a biocompatible carrier in future clinical applications.

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