alexa Metabolic Control of the TCA cycle by the YdcI Transcriptional Regulator in Escherichia coli | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Metabolic Control of the TCA cycle by the YdcI Transcriptional Regulator in Escherichia coli

Yousuke Nishio1*, Tomoko Suzuki2, Kazuhiko Matsui3 and Yoshihiro Usuda2

1Institute for Innovation, Ajinomoto Co, Inc, 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan

2Research Institute for Bioscience Products & Fine Chemicals,Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan

3Corporate Planning Department, Ajinomoto Co., Inc., 15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan

*Corresponding Author:
Yousuke Nishio
Institute for Innovation
Ajinomoto Co., Inc
1-1 Suzuki-cho, Kawasaki-ku
Kawasaki 210-8681, Japan
E-mail: [email protected]

Received date: June 10, 2013; Accepted date: June 27, 2013; Published date: July 01, 2013

Citation: Nishio Y, Suzuki T, Matsui K, Usuda Y (2013) Metabolic Control of the TCA cycle by the YdcI Transcriptional Regulator in Escherichia coli. J Microb Biochem Technol 5:059-067. doi:10.4172/1948-5948.1000101

Copyright: © 2013 Nishio Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Understanding the regulation and control of the expression of genes encoding metabolic enzymes is crucial for production using microbes. To overcome technical difficulties involved in identifying regulatory network systems, we designed a DNA motif finding procedure combining transcriptome and genome sequence data. Here, we used the ArcAB two-component system of Escherichia coli, which controls genes involved in the TCA cycle and energy metabolism, as a model to identify DNA motifs involved in gene-expression regulation. DNA-array data were used to extract up-regulated genes from ΔarcA and ΔarcB E. coli strains, and the upstream sequences were subjected to DNA-motif finding. Sequence similarity and conserved residues identified the known ArcA-binding motif and a novel DNA-motif candidate that was estimated to be related to YdcI, a putative LysR-type transcriptional regulator. A hypothetical YdcI-binding motif was found upstream of the gltA gene, suggesting that YdcI might control the carbon flux into the TCA cycle. To verify this, l-glutamic-acid production and citrate synthase activity in the ydcI gene-amplified strain were investigated. Our findings suggested that YdcI is a transcription factor that regulates the expression of gltA and other genes, and controls the carbon flux into the TCA cycle.

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