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ISSN: 2153-0637

Journal of Glycomics & Lipidomics
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Research Article

Metabolic Profiling and Quantification of Sphingolipids by Liquid Chromatography-Tandem Mass Spectrometry

Wujuan Zhang1, Brian Quinn2, Sonya Barnes2, Gregory A Grabowski2,3, Ying Sun2,3 and Kenneth Setchell1*,3

1TheDepartment of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA

2TheDivision of Human Genetics, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA

3The Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA

*Corresponding Author:
Kenneth Setchell
Cincinnati Children’s Hospital Medical Center The Department of Pathology and Laboratory Medicine
3333 Burnet Avenue, MLC 7019, Cincinnati, Ohio, USA
Tel: (513) 636-4548
Fax: (513) 636-7853
E-mail: [email protected]

Received date: August 26, 2013; Accepted date: October 19, 2013; Published date: October 20, 2013

Citation: Zhang W, Quinn B, Barnes S, Grabowski GA, Sun Y, et al. (2013) Metabolic Profiling and Quantification of Sphingolipids by Liquid Chromatography-Tandem Mass Spectrometry. J Glycomics Lipidomics 3:107. doi:10.4172/2153-0637.1000107

Copyright: © 2013 Zhang W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A precise, robust, and specific liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS)
method was developed for profiling and quantifying glucosyl sphingosine (GS), glucosylceramide (GC), ceramide (Cer),
lactosylceramide (LacCer) and sulfatide lipid species in a variety of mouse tissues. The linear response ranges of these
species were 0.05-25 ng. The major GC species identified in visceral tissues of mice were GCs with N-acyl chains of
C24-1, C24, C22, C16 lengths, but the qualitative and quantitative profiles differed among tissues. GC levels in spleen
were approximately 3-5 times higher than in liver and lung. Brain differed from visceral tissues in that galactosylceramides
(GalCer) were the predominant monohexosylceramide species identified. A silica column used in hydrophobic interaction
liquid chromatography (HILIC) mode was capable of differentiating GC and GalCer. The analysis of mouse brain samples
revealed that GC accounted for only 0.3% of the total monohexosylceramides. Cer and LacCer were also profiled and
quantified in mouse brain, lung, liver and spleen. Application of these methods greatly facilitated a range of targeted
sphingolipidomic investigations and will permit a better understanding of the function and mechanism of these diverse
molecular species in various disease animal models, including Gaucher disease.

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