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Metabolic Profiling Comparison of Human Pancreatic Ductal Epithelial Cells and Three Pancreatic Cancer Cell Lines using NMR Based Metabonomics | OMICS International | Abstract
ISSN-2155-9929

Journal of Molecular Biomarkers & Diagnosis
Open Access

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Research Article

Metabolic Profiling Comparison of Human Pancreatic Ductal Epithelial Cells and Three Pancreatic Cancer Cell Lines using NMR Based Metabonomics

Miki Watanabe1, Sulaiman Sheriff2, Kenneth B. Lewis1, Junho Cho1, Stuart L. Tinch1 Ambikaipakan Balasubramaniam2,3,4 and Michael A. Kennedy1*

1Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA

2Department of surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA

3Shriners Hospital for Children, Cincinnati, OH 45229, USA

4Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220, USA

*Corresponding Author:
Michael A. Kennedy
Miami University, 106 Hughes Laboratories
Department of Chemistry and Biochemistry
Miami University, Oxford, OH 45056, USA
Tel: 513-529-8267
E-mail: [email protected]

Received date: November 01, 2011; Accepted date: March 06, 2012; Published date: March 12, 2012

Citation: Watanabe M, Sheriff S, Lewis KB, Cho J, Tinch SL, et al. (2012) Metabolic Profiling Comparison of Human Pancreatic Ductal Epithelial Cells and Three Pancreatic Cancer Cell Lines using NMR Based Metabonomics. J Mol Biomark Diagn S3:002. doi:10.4172/2155-9929.S3-002

Copyright: © 2012 Watanabe M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Abstract

Metabolic profiles of hydrophilic and lipophilic cell extracts from three cancer cell lines, Miapaca-2, Panc-1 and AsPC-1, and a non-cancerous pancreatic ductal epithelial cell line, H6C7, were examined by proton nuclear magnetic resonance spectroscopy. Over twenty five hydrophilic metabolites were identified by principal component and statistical significance analyses as distinguishing the four cell types. Fifteen metabolites were identified with significantly altered concentrations in all cancer cells, e.g. absence of phosphatidylgrycerol and phosphatidylcholine, and increased phosphatidylethanolamine and cholesterols. Altered concentrations of metabolites involved in glycerophospholipid metabolism, lipopolysaccharide and fatty acid biosynthesis indicated differences in cellular membrane composition between non-cancerous and cancer cells. In addition to cancer specific metabolites, several metabolite changes were unique to each cancer cell line. Increased N-acetyl groups in AsPC-1, octanoic acids in Panc-1, and UDP species in Miapaca-2 indicated differences in cellular membrane composition between the cancer cell lines. Induced glutamine metabolism and protein synthesis in cancer cells were indicated by absence of glutamine other metabolites such as acetate, lactate, serine, branched amino acids, and succinate. Knowledge of the specifically altered metabolic pathways identified in these pancreatic cancer cell lines may be useful for identifying new therapeutic targets and studying the effects of potential new therapeutic drugs.

Keywords

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