alexa Metabolic Profiling of Dunaliella salina Shifting Culti
ISSN: 2153-0769

Metabolomics:Open Access
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Research Article

Metabolic Profiling of Dunaliella salina Shifting Cultivation Conditions to Nitrogen Deprivation

Lv H1,2*, Cui X2, Wang S2 and Jia S2*

1Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin, 300457, P.R. China

2Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Lab of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin, 300457, P.R. China

*Corresponding Author:
Lv H
Tianjin Key Laboratory of Marine Resources and Chemistry
Tianjin University of Science and Technology
Tianjin, 300457, P.R. China
E-mail: [email protected] and

Jia S Key Laboratory of Industrial Fermentation Microbiology
Ministry of Education
Tianjin Key Lab of Industrial Microbiology
Tianjin University of Science and Technology
Tianjin, 300457, P.R. China
Tel: +86-22-60601598
Fax: 86-22-60602298
E-mail: [email protected]

Received Date: January 29, 2016; Accepted Date: February 18, 2016; Published Date: February 20, 2016

Citation: Lv H, Cui X, Wang S, Jia S, (2016) Metabolic Profiling of Dunaliella salina Shifting Cultivation Conditions to Nitrogen Deprivation. Metabolomics 6:170. doi: 10.4172/2153-0769.1000170

Copyright: © 2016 Lv H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Dunaliella salina is a model chlorophyte microalga in studying carotenoid metabolism and osmoregulation. Nitrogen depletion plays an important role in inducing β-carotene accumulation of D. salina cells. However, the underlying mechanism is still unclear. In this study, the metabolic profiling of D. salina cells during the process of nitrogen deprivation was performed by gas chromatography-mass spectrometry. A total of 78 metabolites were identified and quantified, including 16 amino acids, 19 sugars, 13 organic acids, 11 alcohols, 11 fatty acids, 4 amines and 4 others. Nitrogen deprivation induced changes in saturation of fatty acids by increasing saturated fatty acids and decreasing polyunsaturated fatty acids in D. salina. The relative levels of metabolites such as sucrose, maltose and organic acids, which act as carbon skeleton, were increased, in contrast, glycerol, myo-inositol which are related to the osmoregulatory mechanism, kept constant under nitrogen deprivation condition. The levels of proline, glutamic acid and other metabolites, which were involved in protective function, were increased in nitrogen deprivation condition. The precursors of these metabolites are the intermediates of the Calvin cycle and the TCA cycle. The results of this research might be applicable to the interpretation of metabolic synthesis and fatty acid production during process of nitrogen deprivation and provide a new method for accumulating high amounts of carotenoids in D. salina.


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