Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics InvestigationHu JZ1*, Wang X1,2, Feng J1, Robertson BJ1, Waters KM1, Tilton SC1, Pounds JG1, Corley RA1, Liu M2 and Hu M1
- *Corresponding Author:
- Hu JZ
Senior Scientist, Pacific Northwest National Laboratory
Richland, WA, USA
Tel: 509- 371-6544
E-mail: [email protected]
Received Date: April 14, 2015; Accepted Date: May 05, 2015; Published Date: May 12, 2015
Citation: Hu JZ, Wang X, Feng J, Robertson BJ, Waters KM, et al. (2015) Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics Investigation. Metabolomics 5:143. doi: 10.4172/2153-0769.1000143
Copyright: © 2015 Hu JZ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
1H-NMR metabolomics was used to investigate the changes of metabolites in the lungs of mice with and without being exposed to a controlled amount of cigarette smoke. It was found that the concentrations of adenosine derivatives (i.e. ATP, ADP and AMP), inosine and uridine were significantly changed in the lungs of mice exposed to cigarette smoke when compared with controls regardless the mice were obese or of regular weight. The decreased ATP, ADP, AMP and elevated inosine suggested that the deaminases in charge of adenosine derivatives to inosine derivatives conversion would be significantly changed in the lungs of mice exposed to cigarette smoke. Indeed, transcriptional study confirmed that the concentrations of adenosine monophosphate deaminase 2 and adenosine deaminase 2 were significantly changed in the lungs of mice exposed to cigarette smoke. We also found that the ratio of glycerophosphocholine (GPC) to phosphocholine (PC) was significantly increased in the lungs of obese mice compared with those of the regular weight mice. The GPC/PC ratio was further elevated in the lungs of obese group exposed to cigarette smoke.