alexa Methanolic Extract of Wedelia trilobata in Antiprolifer
ISSN: 2329-6836

Natural Products Chemistry & Research
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Research Article

Methanolic Extract of Wedelia trilobata in Antiproliferation and Apoptotic Activity

Uday Venkatesh1, Shiva Prasad Kollur2*, Chethan Javarashetty3, Shankar Jayarama4 and Satish Kumar Murari5*
1School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India
2Manipal Centre for Natural Sciences, Manipal University, Udupi-576 104, Karnataka, India
3Central Coffee Research Institute, Balehonoor, Chikkamagalur-577 117, Karnataka, India
4Department of Biotechnology, Teresian College, Siddarthanagar, Mysore-570 019, Karnataka, India
5Department of Chemistry, PES College of Engineering, Mandya-571 401, Karnataka, India
*Corresponding Author : Shiva Prasad Kollur
Manipal Centre for Natural Sciences, Manipal University
Udupi-576 104, Karnataka, India
E-mail: [email protected]
  Satish Kumar Murari
Department of Chemistry, P.E.S. College of Engineering
Mandya-571 401, Karnataka, India
E-mail: [email protected]
Received: February 25, 2016; Accepted: March 03, 2016; Published: March 10, 2016
Citation: Venkatesh U, Kollur SP, Javarashetty C, Jayarama S, Murari SK (2016) Methanolic Extract of Wedelia trilobata in Antiproliferation and Apoptotic Activity. Nat Prod Chem Res 4:210. doi:10.4172/2329-6836.1000210
Copyright: © 2016 Venkatesh U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Herein, we report extraction of biologically important isolates from medicinally valuable plant Wedelia trilobata. The biological activities viz., anti-proliferative activity and cytotoxic effect of the methanolic extract of Wedelia trilobata was investigated by Thymidine uptake assay, wound healing assay and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, respectively. In addition, apoptotic activity was also examined through nuclear staining and DNA fragmentation assays. The IC50 value of the methanolic extract for the growth inhibition of MEG-01 cells was found to be 80 μg/ml under which the normal HEK-293 cells were resistant to the toxic effect. Concentration and time dependent studies in Thymidine uptake and wound healing assays revealed the prominent antiproliferative activity against MEG-01 at the concentration of 80 μg/ml for 48 h. The nuclear staining and DNA fragmentation assays also confirmed that the methanolic extract induces apoptosis in MEG-01 cells.

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