Method Development and Validation for Simultaneous Estimation of Dexlansoprazole and Meloxicam by Rp-Hplc
Jalli Sriharsha*, Srinivasa Murthy M, Bharat Kumar D, Sravan K, Shiva kumar P, Shirisha A and Pranusha K
Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda
- *Corresponding Author:
- Sri Harsha Jalli
Vignan Institute of pharmaceutical sciences
E-mail: [email protected]
Received date: April 08, 2015; Accepted date: May 11, 2015; Published date: May 18, 2015
Citation: Sriharsha J, Srinivasa Murthy M, Bharat Kumar D, Sravan K, Shiva kumar P, et al. (2015) Method Development and Validation for Simultaneous Estimation of Dexlansoprazole and Meloxicam by Rp-Hplc. Pharm Anal Acta 6:370. doi: 10.4172/2153-2435.1000370
Copyright: ©2015 Sriharsha J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
To develop a method for simultaneous estimation of Dexlansoprazole and Meloxicam by RP-HPLC and validate the developed method according to ICH guidelines. The column used for method development of Dexlansoprazole and Meloxicam was Hypersil-BDS, C18, 250*4.6 mm, 5μ. Methanol and Acetonitrile were used in the ration of 60:40 for the method development. The developed method was validated for various parameters such as specificity, linearity, range, accuracy, precision, system suitability, robustness, ruggedness etc. The method was developed and optimized method was chosen and validated and the results were tabulated according to ICH guidelines. The result obtained in this study demonstrated that the HPLC method described is specific, accurate, precise, linear, ruggedness, robustness and stability indicating for the simultaneous determination of Dexlansoprazole and Meloxicam.