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Tengli AR* and Gurupadayya BM
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, S.S. Nagar, Mysore-570015, India
Received Date: March 28, 2013; Accepted Date: April 27, 2013; Published Date: April 3, 2013
Citation: Tengli AR, Gurupadayya BM (2013) Method Development and Validation of Tablet Dosage form Containing Losartan, Atenolol and Hydrochlorthiazide Using Internal Standard by RP-HPLC. J Chromat Separation Techniq 4: 180. doi: 10.4172/2157-7064.1000180
Copyright: © 2013 Tengli AR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple, sensitive and specific RP-HPLC method with UV detection was developed for the simultaneousestimation of losartan, atenelol and hydrochlorothiazide in tablet dosage form and telmisartan as an internal standard. Separation was achieved with an phenomenex luna 5 μ CN 100R, 250×4.60 mm 5 micron size column, ambient temperature with a low pressure gradient mode with mobile phase containing acetonitril, and 0.2% of diammonium hydrogen orthophosphate buffer pH 2.8 adjusted with orthophosphoric acid (50:50). The flow rate was 1 mL min-1 and eluent was monitored at 230 nm. The selected chromatographic conditions were found to effectively separate losartan (LOSAT), atenelol (ATEN) and hydrochlorothiazide (HCT) with retention time of 6.0, 5.0 and 4.0 min respectively. The proposed method was found to be rectilinear over the range of 25-150 μgmL-1, 25-150 μgmL-1 and 6.25-37.50 μgmL-1 for losartan, atenelol and hydrochlorothiazide respectively. The proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined pharmaceutical dosage forms