alexa Method Validation for the Trace Analysis of Geosmin and 2-Methylisoborneol in Water by and#195;and#162;and#194;and#8364;and#194;and#339;Salt-Freeand#195;and#162;and#194;and#8364;and#194;and#157; Purge-and-Trap Sampling/GC-MS, Using the Eclipse 4660 Sample Concentrator | Abstract
ISSN: 2157-7587

Hydrology: Current Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Method Validation for the Trace Analysis of Geosmin and 2-Methylisoborneol in Water by “Salt-Free” Purge-and-Trap Sampling/GC-MS, Using the Eclipse 4660 Sample Concentrator

Thavrin Manickum* and Wilson John
Scientific Services Laboratories, Engineering & Scientific Services, Heald Office, Level 3, Umgeni Water, 310 Burger Street, Pietermaritzburg 3201, KwaZulu-Natal, South
Corresponding Author : Thavrin Manickum
Scientific Services Laboratories
Engineering & Scientific Services
Head Office, Level 3, Umgeni Water
310 Burger Street, Pietermaritzburg 3201
KwaZulu-Natal, South Africa
Tel: +27 33 341 1067
Fax: +27 33 341 1501
E-mail: [email protected]
Received March 27, 2012; Accepted May 23, 2012; Published May 30, 2012
Citation: Manickum? T, John W (2012) Method Validation for the Trace Analysis of Geosmin and 2-Methylisoborneol in Water by “Salt-Free” Purge-and-Trap Sampling/GC-MS, Using the Eclipse 4660 Sample Concentrator. Hydrol Current Res 3:134. doi: 10.4172/2157-7587.1000134
Copyright: © 2012 Manickum? T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google


The Eclipse 4660 purge-and trap sample concentrator, coupled to a gas chromatograph-mass spectrometer, was fully validated for trace analysis of the taste-odorants geosmin (GSM) and 2-methylisoborneol (2-MIB) in the absence of salt or methanol. The procedure involved purging a 25 mL sample volume, at 80°C, for 11 min. Quantitation was done by separation on an HP 5-MS capillary column (30 m × 0.25 mm × 0.25 μm), followed by mass spectrometric detection in the selected ion monitoring (SIM) mode. The method was found to be linear (r2 ≥ 0.995) over the calibration range 0-100 ng/L for both target analytes. The overall accuracy, determined by recovery, was 104.21% ± 19.46 (mean ± SD, range = 65.29-143.13%) for 2-MIB, and 85.24% ± 8.28 (mean ± SD, range = 68.66-101.82%) for GSM. The within-day precision averaged 7.97% for 2-MIB, and 8.77% for GSM. The day-to-day precision was 20.96% for 2-MIB, and 7.52% for GSM. The limit of quantitation (LOQ) was 6 ng/L and 5 ng/L for 2-MIB and GSM; the limit of detection (LOD) was 5 ng/L and 4 ng/L for 2-MIB and GSM respectively. Earlier findings regarding the dehydration of 2-MIB to 2-methyl-2-bornene (2-M-2-B) were confirmed. The average amount of 2-M-2-B formed was 27% (range: 15-50%), with RSD = 33.26%. There is evidence of dehydration of 2-MIB to 2-M-2-B at sample purge temperature of 40°C and higher. The poor day-to-day precision for 2-MIB has been proposed to be due to the inconsistent amount of 2-M-2-B formed. A minimum sample purge temperature of 40°C is required to purge 2-MIB and GSM from water, which can be detected by GC/MS under the purge-and-trap conditions at a concentration of 100 ng/L. The developed method was found to be sensitive, accurate, precise and reproducible for trace analysis of geosmin in water matrices.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A


b[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A


[email protected]

1-702-714-7001Extn: 9037


James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals


Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T


[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version