Methylation Profile of CD4+ T Cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis
|Ekua W Brenu1,2*, Donald R Staines2 and Sonya M Marshall-Gradisnik1,2|
|1School of Medical Science, Griffith Health Centre, Griffith University, Gold Coast, QLD, Australia|
|2The National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Australia|
|Corresponding Author :||Ekua Weba Brenu
Griffith University, Parklands Drive
Griffith Health Centre
National Centre for Neuroimmunology and Emerging Diseases
Southport, QLD, 4215, Australia
E-mail: [email protected]
|Received March 28, 2014; Accepted June 13, 2014; Published June 20, 2014|
|Citation: Brenu EW, Staines DR, Marshall-Gradisnik SM (2014) Methylation Profile of CD4+ T Cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis. J Clin Cell Immunol 5:228. doi: 10.4172/2155-9899.1000228|
|Copyright: © 2014 Brenu EW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Objective: Methylation is known to regulate biological processes and alterations in methylation patterns have been associated with a variety of diseases. Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an unexplained disorder associated with immunological and molecular changes. CD4+T cells specifically, regulatory T cells (Tregs) have been implicated in CFS/ME patients where significant increases in Tregs have been observed in these patients. Therefore the objective of this study was to examine methylation in CD4+T cells from CFS/ME patients.
Methods: The study comprised twenty-five CFS/ME participants and eighteen controls aged between 25-60 years. A volume of 20 ml whole blood was collected from each participant and peripheral blood mononuclear cells were isolated via density gradient centrifugation. A negative isolation kit was used to isolate the CD4+T cells from the peripheral blood samples. Genome wide methylation studies were performed on isolated CD4+T cells using the Illumina Infinium 450 K Human methylation array system. Data analysis was performed using Genome studio and Partek Enrichment software.
Results: 120 CpGs were observed to be differentially methylated in the CFS/ME patients in comparison to the controls. Of these 70 were associated with known genes. The majority of the differential methylated regions in the CFS/ME patients were hypomethylated. Additionally, most of the genes with differentially methylated regions in the CFS/ME patients were responsible for apoptosis, cell development, cell function and metabolic activity.
Conclusion: The present study demonstrates that epigenetic changes in CD4+T cells may have a potential role in the immunological changes observed in CFS/ME patients.