Methyl-ÃÂ-Cyclodextrin /CTAB Sensitized Fluorescence Method for the Determination of Levofloxacin
Qiuye Ren and Xiashi Zhu*
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China
- *Corresponding Author:
- Xiashi Zhu
College of Chemistry & Chemical Engineering
Yangzhou University, Yangzhou 225002, PR China
E-mail: [email protected], [email protected]
Received Date: May 16, 2015; Accepted Date: May 28, 2015; Published Date: June 04, 2015
Citation: Qiuye Ren, Xiashi Zhu (2015) Methyl-Β-Cyclodextrin /CTAB Sensitized Fluorescence Method for the Determination of Levofloxacin. Pharm Anal Acta 6:376. doi: 10.4172/2153-2435.1000376
Copyright: © 2015 Qiuye Ren, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this work, the method of methyl-β-cyclodextrin (methyl-β-CD) and cetyltrimethyl ammonium bromide (CTAB) cooperatively sensitized fluorescence analysis to determine levofloxacin (LVFX) was developed. The results were shown that, in the system of methyl-β-cyclodextrin-CTAB, the fluorescence intensity of LVFX increased a lot than in H2O medium. Under the conditions of λex/em=330/507 nm and pH 4.5, the linear range and the detection limit for LVFX were found to be 0.04 ~ 4.0 μg/mL and 0.316 ng/mL, respectively. The mechanism of sensitized fluorescence method was discussed by the Benesi-Hildebrand method and the fluorescence quantum yield of fluorescence. The proposed method has been applied for the determination of LVFX in eye drops real samples with satisfactory recovery.