Micellar Effects of Cetyl Pyridinium Chloride on Antioxidant Capacity,Voltammetric Response of Serum and Saliva Samples
- *Corresponding Author:
- Armindo Antonio Alves
Biochemistry Department, Biology Institute
Campinas State University, Brazil
Fax: 55- 19-3521-6129
E-mail: [email protected]
Received Date: April 24, 2012; Accepted Date: November 05, 2012; Published Date: November 07, 2012
Citation: de Passos MA, de Macedo DV, de Cássia Silva Luz R, Nunes LAS, Kubota4 LT, et al. (2012) Micellar Effects of Cetyl Pyridinium Chloride on Antioxidant Capacity, Voltammetric Response of Serum and Saliva Samples. J Biosens Bioelectron 3:130. doi: 10.4172/2155-6210.1000130
Copyright: © 2012 de Passos MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The production of reactive oxygen species due to increased energy demand during physical exercise can increase the cellular and blood oxidation state. The reducing power of biological samples reflects its antioxidant capacity, largely maintained by Low Molecular Weight Antioxidants (LMWA), which donate electrons to radical species. LMWA include antioxidants such as uric acid, vitamins C and E and lipoic acid, among others. The electroanalytical technique of Differential Pulse Voltammetry (DPV) presents a good methodological alternative to quantify acute and chronic modulations of the antioxidant capacity from biological fluids in response to metabolic adaptations caused by physical exercise. However, when biological samples are analyzed, proteins are an important preanalytical interfering in the technique. The proteins can be adsorbed on the electrode surface during the potential application, resulting in a significant decrease of voltammetric signal. The aim of the present study was to investigate
the applicability of cationic surfactant Cetyl Pyridinium Chloride (CPC) as a micellar system for the improvement of DPV technique for serum and saliva analysis. Forty individuals' samples were analyzed. The obtained data revealed that the use of CPC increased the sensitivity and stability of the voltammetric signal, enabling the application of the method DPV for serum and saliva samples. Our data suggest that the voltammetric signal of samples is influenced mainly by the uric acid concentration.