alexa Micellar Liquid Chromatographic Determination of Idrocilamide in Dosage Form and Biological Fluids: Application to Stability Study | OMICS International | Abstract
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Micellar Liquid Chromatographic Determination of Idrocilamide in Dosage Form and Biological Fluids: Application to Stability Study

Fathalla Belal*, Manal Eid, Amina EL-Brashy and Wael Talaat

Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt

*Corresponding Author:
Fathalla Belal
Department of Analytical Chemistry
Faculty of Pharmacy, University of Mansoura
Mansoura 35516, Egypt
Tel: +966 14677348
E-mail: [email protected]

Received Date: December 12, 2014; Accepted Date: January 27, 2015; Published Date: February 02, 2015

Citation: Belal F, Eid M, EL-Brashy A, Talaat W (2015) Micellar Liquid Chromatographic Determination of Idrocilamide in Dosage Form and Biological Fluids:Application to Stability Study. Pharm Anal Acta 6:335. doi: 10.4172/2153-2435.1000335

Copyright: ©2015 Belal F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple, stability-indicating, reversed phase high perfomance liquid chromatographic method was developed and validated for the determination of idrocilamide in the presence of its degradation products .The separation was conducted using A Hibar C18 (150 × 4.6 mm i.d ) stainless steel column at ambient temperature with UV detection at 277 nm . Micellar mobile phase consisted of 0.1 M sodium dodecyl sulphate, 10% n-propanol, 0.3 % triethylamine in 0.02 M phosphoric acid (pH 6) was used and pumped at a flow rate of 1 mL/min .The calibration curve was rectilinear over the concentration range of 1-10 μg/mL with a detection limit of 0.1 μg /mL and quantification limit of 0.3 μg/mL .The proposed method was successfully applied to the analysis of idrocilamide in commercial cream with mean % recovery of 100.74 ± 0.93. The method was extended to the in-vitro determination of idrocilamide in spiked human plasma and urine samples with mean % recoveries of 99.93 ± 0.31 and 100.1 ± 0.26 respectively Moreover, the method was utilized to investigate the kinetics of both acid and alkaline induced degradation of the drug . The apparent first –order rate constant, half life time and activation energies of the degradation reactions were calculated

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version