Microbial Diversity in Continuous Flow Constructed a Wetland for the Treatment of Swine Waste
- *Corresponding Author:
- Abasiofiok Mark Ibekwe
USDA-ARS-U. S. Salinity Laboratory
450 W Big Springs Rd, Riverside CA 92507
Email: [email protected]
Received Date: June 12, 2017 Accepted Date: June 16, 2017 Published Date: July 22, 2017
Citation: Ibekwe AM, Ma J, Murinda S, Reddy GB (2017) Microbial Diversity in Continuous Flow Constructed a Wetland for the Treatment of Swine Waste. Hydrol Current Res 8: 277. doi: 10.4172/2157-7587.1000277
Copyright: © 2017 Ibekwe AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Contaminant removal may largely be a function of many microbial processes in constructed wetlands. However, the role of microbial diversity for the removal of swine waste in constructed wetlands is limited. Here, we used 454/ GS-FLX pyrosequencing to assess archaeal, bacterial, and fungal composition within a surface flow constructed wetland to determine their spatial dynamics and contaminant removal within the wetland. We analyzed our data using UniFrac and principal coordinate analysis (PCoA) to compare community structure and specific functional groups of bacteria, archaea, and fungi in different sections of the wetland. PCoA analysis showed that, bacterial, archaeal, and fungal composition were significantly different (p=0.001) for the influent compared to the final effluent. Our results showed that, the wetland system contained relatively higher proportions of bacteria and fungi than archaea. Most of the bacteria and archaea that were associated with nitrogen removal were affiliated with Nitrosomonas which are ammonia oxidizing bacteria (AOB), Candidatus Solibacter, an anaerobic ammonia oxidizing bacteria (Anammox), as well as Nitrosopumilus, ammonia oxidizing archaea (AOA). The detection of AOB, Anammox, and AOA in this wetland shows abundance and diversity of these microorganisms that are responsible for nitrification processes in constructed wetlands.