MicroRNA Analysis in Human Papillomavirus (HPV)-Associated Cervical Neoplasia and Cancer
- *Corresponding Author:
- Saleem A. Khan, PhD
Department of Microbiology and Molecular Genetics
University of Pittsburgh School of Medicine
450 Technology Drive, Bridgeside Point 2
Room 520, Pittsburgh
E-mail: [email protected]
Received date: Janurary 05, 2011; Accepted date: February 02, 2011; Published date: February 04, 2011
Citation: McBee WC Jr, Gardiner AS, Edwards RP, Lesnock JL, Bhargava R, et al. (2011) MicroRNA Analysis in Human Papillomavirus (HPV)-Associated Cervical Neoplasia and Cancer. J Carcinogene Mutagene 2:114. doi: 10.4172/2157-2518.1000114
Copyright: © 2011 McBee WC Jr, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: MicroRNAs (miRNAs) are ~22 nt single-stranded, non-coding RNAs that generally negatively regulate their target mRNAs at a posttranscriptional level. Differential expression of miRNAs has been observed in many human cancers.
Methods: To study their potential role in the pathogenesis of human papillomavirus (HPV) type 16-associated cervical neoplasia and cancer, we analyzed miRNA expression in cervical tissue from the normal cervix, moderate/ severe dysplasia, and invasive squamous cell carcinoma.
Results: Using RNA from six cervical cancers, three dysplasias, and four normal samples and the TaqMan® MicroRNA Arrays, we found that 18 miRNAs were overexpressed and 2 underexpressed in cervical cancer compared to normal cervical tissue. We further demonstrated via individual TaqMan® MicroRNA Assays that 8 miRNAs (miRs- 16, 21, 106b, 135b, 141, 223, 301b, and 449a) were significantly overexpressed and 2 miRNAs (miRs-218 and 433) were significantly underexpressed in cervical cancer compared to normal cervical tissue. MiR-21, miR-135b, miR- 223, and miR-301b were overexpressed in cervical cancer compared to both cervical dysplasia and normal tissue. MiR-218 was similarly underexpressed in cervical cancer compared to dysplasia and normal tissue.
Conclusions: Our results suggest that ten miRNAs can delineate cervical cancer from normal cervical tissue, and five miRNAs may have potential as markers for progression from dysplasia to invasive cervical disease.