alexa Midland Blotting: A Rapid, Semi-Quantitative Method for Biosensor Surface Characterization | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Midland Blotting: A Rapid, Semi-Quantitative Method for Biosensor Surface Characterization

Jo V Rushworth*, Asif Ahmed and Paul A Millner

School of Biomedical Sciences, Faculty of Biological Science, University of Leeds, UK

*Corresponding Author:
Dr. Jo V Rushworth
School of Biomedical Sciences
Faculty of Biological Science
University of Leeds, UK
Tel: 00 44 113 34 37753
E-mail: [email protected]

Received Date: November 26, 2013; Accepted Date: December 19, 2013; Published Date: December 26, 2013

Citation: Rushworth JV, Ahmed A, Millner PA (2013) Midland Blotting: A Rapid, Semi-Quantitative Method for Biosensor Surface Characterization. J Biosens Bioelectron 4:146. doi: 10.4172/2155-6210.1000146

Copyright: © 2013 Rushworth JV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Biosensor performance and readout are critically dependent upon sensor surface characteristics. It is vital that key steps in sensor construction, such as base layer polymer/Self-Assembled Monolayer (SAM) deposition and bioreceptor tethering, are controlled tightly in order to achieve high sensitivity, specificity and reproducibility. Here, we present a rapid, semi-quantitative method by which key biosensor surface features can be characterised using chemiluminescence. This technique, which we have termed midland blotting, permits the detection of biosensor surface components through the attachment of a HorseRadish Peroxidase (HRP)-conjugated reagent to the target of interest. Upon addition of luminol-based substrate, HRP generates a reagent which emits light when it decays. The light signal is proportional to the bound HRP on the sensor surface. We show here that midland blotting allows the measurement and validation of various important surface features including: (1) availability of functional groups on the polymer or SAM layer; (2) bioreceptor tethering and (3) analyte binding. Midland blotting is rapid, cost-effective and allows for much faster optimisation of biosensor surface design. This method also provides a simple way of troubleshooting and can explain sensor performance when combined with readout data. In this report, we have focussed on midland blotting for electrochemical immunosensors as a proof of concept, but this technique is readily applicable to all biosensor systems.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version