alexa Mitochondrial and Nuclear DNA for Identification of Forensically Important Flesh Flies (Sarcophagidae: Boettcherisca Spp)
ISSN: 2161-0983

Entomology, Ornithology & Herpetology: Current Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Mitochondrial and Nuclear DNA for Identification of Forensically Important Flesh Flies (Sarcophagidae: Boettcherisca Spp)

Roziah A1,2*, Tan SH2, Lee HL1 and Mohamed Z2

1Medical Entomology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia

2Unit of Genetics and Molecular Biology, Institute Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

*Corresponding Author:
Roziah A
Medical Entomology Unit, Institute for Medical Research
Jalan Pahang, 50588 Kuala Lumpur, Malaysia
Tel: 603-2616 2666
E-mail: [email protected]

Received date: July 10, 2015; Accepted date: August 21, 2015; Published date: August 25, 2015

Citation: Roziah A, Tan SH, Lee HL, Mohamed Z (2015) Mitochondrial and Nuclear DNA for Identification of Forensically Important Flesh Flies (Sarcophagidae: Boettcherisca Spp). Entomol Ornithol Herpetol 4:163. doi: 10.4172/2161-0983.1000163

Copyright: © 2015 Roziah A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Morphology-based identification of flesh flies (Family: Sarcophagidae) is normally limited to genus level due to their high similarities in characteristics. Even though identification to species level is possible, expertise in taxonomy is still needed; as sarcophagid female flies are extremely difficult to identify. In this study, a molecular approach was used to examine the use of 12S & 16S ribosomal mitochondrial DNA (1172 bp) and Internal Transcribed Spacer (ITS) region of nuclear DNA (~1500 bp) to discriminate four species of the subgenus Boettcherisca namely, Boettcherisca javanica, B. peregrina, B. karnyi and B. highlandica. Neighbour-Joining phylogenetic tree was generated for each gene. Interspecific values calculated using the Kimura-2-parameter distance model were in the low range of 0.5%-1.3% and 0.5%-1.9% for 12S & 16SrDNA and ITS region respectively. However, identifications of B. karnyi and B. peregrina using ITS; and B. peregrina using 12S & 16S alone can be ambiguous. Therefore phylogenetic tree analyses of both genes showed a likely for these specimens to be distinguished and confirmed the potential of these genes as specific sarcophagid identification markers

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords