Mitochondrial and Nuclear DNA for Identification of Forensically Important Flesh Flies (Sarcophagidae: Boettcherisca Spp).Roziah A1,2*, Tan SH2, Lee HL1 and Mohamed Z2
- *Corresponding Author:
- Roziah A
Medical Entomology Unit, Institute for Medical Research
Jalan Pahang, 50588 Kuala Lumpur, Malaysia
Tel: 603-2616 2666
E-mail: [email protected]
Received date: July 10, 2015; Accepted date: August 21, 2015; Published date: August 25, 2015
Citation: Roziah A, Tan SH, Lee HL, Mohamed Z (2015) Mitochondrial and Nuclear DNA for Identification of Forensically Important Flesh Flies (Sarcophagidae: Boettcherisca Spp). Entomol Ornithol Herpetol 4:163. doi: 10.4172/2161-0983.1000163
Copyright: © 2015 Roziah A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Morphology-based identification of flesh flies (Family: Sarcophagidae) is normally limited to genus level due to their high similarities in characteristics. Even though identification to species level is possible, expertise in taxonomy is still needed; as sarcophagid female flies are extremely difficult to identify. In this study, a molecular approach was used to examine the use of 12S & 16S ribosomal mitochondrial DNA (1172 bp) and Internal Transcribed Spacer (ITS) region of nuclear DNA (~1500 bp) to discriminate four species of the subgenus Boettcherisca namely, Boettcherisca javanica, B. peregrina, B. karnyi and B. highlandica. Neighbour-Joining phylogenetic tree was generated for each gene. Interspecific values calculated using the Kimura-2-parameter distance model were in the low range of 0.5%-1.3% and 0.5%-1.9% for 12S & 16SrDNA and ITS region respectively. However, identifications of B. karnyi and B. peregrina using ITS; and B. peregrina using 12S & 16S alone can be ambiguous. Therefore phylogenetic tree analyses of both genes showed a likely for these specimens to be distinguished and confirmed the potential of these genes as specific sarcophagid identification markers.