Mitochondrial Long Chain Fatty Acid Oxidation Related Enzyme Changes in Different Preeclampsia-Like Mouse ModelsXiaoyan Ding, Zi Yang*, Yiwei Han and Huan Yu
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China
- *Corresponding Author:
- Zi Yang
Department of Obstetrics and Gynecology
Peking University Third Hospital, North Garden Rd 49
Haidian district, Beijing 100191, PR China
E-mail: [email protected]
Received Date: June 06, 2014; Accepted Date: July 09, 2014; Published Date: July 14, 2014
Citation: Ding X, Yang Z, Han Y, Yu H (2014) Mitochondrial Long Chain Fatty Acid Oxidation Related Enzyme Changes in Different Preeclampsia- Like Mouse Models. J Hypertens 3:161. doi:10.4172/2167-1095.1000161
Copyright: © 2014 Ding X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Long-chain Free Fatty Acids (FFA) and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) are related to some preeclampsia. We studied the relationship between LCHAD, carnitine palmitoyltransferase (CPT) I and II and FFA in different preeclampsia-like models.
Methods: Classic preeclampsia models with Nw-nitro-L-arginine-methyl ester (L-NA) or Lipopolysaccharide (LPS) injection, antiphospholipid syndrome model with β2 glycoprotein I (β2GPI) injection, and ApoC3 mice with abnormal fatty acid metabolism (ApoC3+NS and ApoC3+L-NA) were used. Controls were saline injected. At days 14 and 18 of pregnancy, serum FFA levels were compared and placental and hepatic LCHAD, CPT I and II mRNA and protein were detected using real-time quantitative PCR and western blot.
Results: Serum FFA levels increased significantly except in LPS, compared to controls (P<0.05). CPT I mRNA and protein increased significantly in the liver and placenta of L-NA and β2GPI and liver of ApoC3+L-NA (P<0.05). CPT II mRNA and protein increased significantly in the liver and placenta of L-NA and the liver of ApoC3+L-NA and β2GPI (P<0.05). LCHAD mRNA and protein increased significantly in the liver and placenta of ApoC3+NS, ApoC3+L-NA and β2GPI, and decreased significantly in L-NA (P<0.05). Serum FFA levels positively correlated with liver CPT I mRNA and protein in all groups (P<0.05) and negatively correlated with liver LCHAD mRNA and protein expression in all groups except in β2GPI group (P<0.05).
Conclusions: Changes and correlations in FFA levels and LCHAD, CPT I and II were different according to preeclampsia-like models. β-oxidation disorders may occur at different stages.