alexa Mobile Automatic Detection System for Bacillus anthracis using Electrochemical DNA Chip | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
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Mobile Automatic Detection System for Bacillus anthracis using Electrochemical DNA Chip

Jun Okada1*, Hideki Horiuchi1, Koji Hashimoto1, Daiji Hirosawa1, Yohei Kurosaki2,3, Keiko Kawamoto4, Jiro Yasuda2,3, Souichi Makino4, Nobuhiro Gemma1 and Masaru Nikaido1

1Toshiba Corporation, 1, Komukai-Toshiba-cho, Saiwai-ku, Kawasaki 212-8582, Japan

2National Research Institute of Police Science (NRIPS), 6-3-1, Kashiwanoha, Kashiwa 277-0882, Japan

3Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan

4Obihiro University of Agriculture and Veterinary Medicine (OUAVM), Inada-cho, Obihiro 080-8555, Japan

*Corresponding Author:
Jun Okada
Toshiba Corporation
1, Komukai-Toshibacho
Saiwai-ku, Kawasaki 212-8582, Japan
Tel: +81-44-549-2044
Fax: +81-44-549-2426
E-mail: [email protected]

Received Date: August 28, 2012; Accepted Date: October 16, 2012; Published Date: October 18, 2012

Citation: Okada J, Horiuchi H, Hashimoto K, Hirosawa D, Kurosaki Y, et al. (2012) Mobile Automatic Detection System for Bacillus anthracis using Electrochemical DNA Chip. J Biosens Bioelectron 3:126. doi: 10.4172/2155-6210.1000126

Copyright: © 2012 Okada J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A novel detection system for biological agents using Loop-Mediated Isothermal Amplification (LAMP) and an electrochemical DNA chip, Bio BulwarkTM, has been developed. Genomic DNAs/RNAs roughly extracted from biological agents were amplified by the LAMP method using target-specific primers and strand-displace polymerase. The amplified DNAs were processed to hybridization with the electrochemical DNA chip. Anodic current derived from hoechst 33258, an electrochemically active intercalator, was relative to the amount of the target DNA hybridized with target-specific probes on the DNA chip. All reactions and measurements were conducted using disposable cassettes, which included the reagents for amplification and the DNA chip, and a mobile automatic detection system. The preliminary study showed that accurate detection of B. anthracis was carried out within 70 minutes. This system would prevent or minimize damage in the event of a criminal attack involving a biological threat.

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