alexa Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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Research Article

Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi

Guo Qiao1,4, Hua Li2, De-Hai Xu3 and Soo II Park1*

1Department of Aquatic Life Medicine, Pukyong National University, Busan 608737, Republic of Korea

2Key Laboratory of Mariculture & Biotechnology, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, Liaoning Province, China

3US Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL 36832, USA

4West Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Chungnam 357945, Republic of Korea

*Corresponding Author:
Soo II Park
Department of Aquatic Life Medicine
Pukyong National University
Busan 608737, Republic of Korea
Tel: +82 51 629 5939
Fax: +82 51 629 5938
E-mail: [email protected]

Received date: January 19, 2012; Accepted date: February 14, 2012; Published date: February 20, 2012

Citation: Qiao G, Li H, Xu DH, Il Park S (2012) Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi. J Bacteriol Parasitol 3:130 doi:10.4172/2155-9597.1000130

Copyright: © 2012 Qiao G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Vibrio scophthalmi has been considered as an opportunistic pathogen of the flat fish. There is little information available on V. scophthalmi adhesion to the host, an important step in the initial infection process. The objectives of this study were to (1) develop a modified Microbial Adherence to Hydrocarbons (MATH) assay using Colony Forming Unit (CFU) method and evaluate the cell surface hydrophobicity of V. scophthalmi , (2) detect slime layer and biofilm production of V. scophthalmi and (3) investigate the motility and antibiotic susceptibility associated with biofilm production of V. scophthalmi . The results showed that cell surface hydrophobicity of V. scophthalmi was moderate by Salt Aggregation Test (SAT) and MATH assay. The fraction partitioned to the hydrocarbon phase (%Adh) was determined significantly higher by modified CFU MATH assay than that by classical spectrophotometer MATH assay and microscope MATH assay. V. scophthalmi produced slime layer and biofilm. The optimal biofilm production conditions for V. scophthalmi were: BHIB media or TSB supplemented with 2% NaCl and 0-0.5% glucose and incubation for 24 h. The level of biofilm production is related with the pathogenicity of V. scophthalmi and antibiotics susceptibility. V. scophthalmi was motile with flagellum-mediated swimming and type IV pilus-mediated twitching, but no swarming.

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