Modified Cas (CRISPR-associated proteins) for Genome Editing and BeyondTingfang Mei, Chu-Jun Liu, Jinhua Yang, Lude Tai and Ling Zhao*
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China
- *Corresponding Author:
- Ling Zhao
State Key Laboratory of Ophthalmology
Zhongshan Ophthalmic Center, Sun Yat-Sen University
54 Xianlie South Rd, Guangzhou, China
E-mail: [email protected]
Received date: July 29, 2016; Accepted date: August 09, 2016; Published date: August 16, 2016
Citation: Mei T, Liu CJ, Yang J, Tai L, Zhao L (2016) Modified Cas (CRISPR-associated Protein) for Genome Editing and Beyond. Adv Tech Biol Med 4:187. doi:10.4172/2379-1764.1000187
Copyright: © 2016 Mei T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
As one of the most popular tools for genome editing, CRISPR/Cas9 system has been widely used in gene targeting, transcriptional regulation, epigenetic modification, even in gene therapy and drug delivery. Although CRISPR/Cas9 system provides a simple, specific and high efficient platform, it still has some limitations. Recently, several modified versions of CRISPR/Cas systems (Cpf1, C2c2, dCas9 and dC2c2) have been remarkably developed for more powerful and customizable genome editing and function studies. In this review, we summarized the key findings and progress on Cas9 and modified Cas proteins, which would help to better understand CRISPR/ Cas systems and advanced future studies on their applications.