Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric AcidKashima Arora1, Monika Tomar2 and Vinay Gupta1*
- *Corresponding Author:
- Vinay Gupta
Department of Physics and Astrophysics
University of Delhi, Delhi 110007, India
Tel: 011 2700 6900
E-mail: [email protected]
Received Date: August 10, 2017; Accepted Date: August 23, 2017; Published Date: August 30, 2017
Citation: Arora K, Tomar M, Gupta V (2017) Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric Acid. J Biosens Bioelectron 8: 246. doi: 10.4172/2155-6210.1000246
Copyright: © 2017 Arora K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A reagentless uric acid biosensor has been realized using Copper implanted tin oxide thin film (Cu:SnO2) based matrix. The biosensing characteristics of implanted matrix have been studied using the electrochemical impedance spectroscopy and cyclic voltammetry. The prepared matrix (Cu:SnO2), because of the presence of Cu, possess redox properties so that the electron transfer from enzyme to the electrode could be accomplished without using any external mediator in the electrolyte. The developed uric acid biosensor exhibits a high sensitivity of about 0.93 mA/ mM and a linear variation in current response over a concentration range from 0.05 to 1.0 mM of uric acid besides high shelf life (~20 weeks). The Michaelis-Menten kinetic parameter (Km) is found to be relatively very low (0.12 mM), which indicates the high affinity of the fabricated bioelectrode towards the uric acid (analyte). The results highlight the importance of implanted Cu:SnO2 thin film as an attractive matrix for the realization of reagentless biosensors towards uric acid.