alexa Modulation of the Intestinal Microbiota and Immune System of Farmed Sparus aurata by the Administration of the Yeast Debaryomyces hansenii L2 in Conjunction with Inulin
ISSN: 2155-9546

Journal of Aquaculture Research & Development
Open Access

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Research Article

Modulation of the Intestinal Microbiota and Immune System of Farmed Sparus aurata by the Administration of the Yeast Debaryomyces hansenii L2 in Conjunction with Inulin

Silvana Teresa Tapia-Paniagua1, Martha Reyes-Becerril2,3, Felipe Ascencio-Valle2, María Ángeles Esteban3, E. Clavijo4, Ma Carmen Balebona1 and Miguel Angel Moriñigo1*

1Group of Prophylaxis and Biocontrol of Fish Diseases, Department of Microbiology, Faculty of Sciences, University of Malaga, 29071-Málaga, Spain

2Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23090, México

3Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain

4Department of Microbiology, Faculty of Medicine, University of Malaga, 29071-Málaga, Spain

*Corresponding Author:
Miguel Angel Moriñigo
Department of Microbiology
Faculty of Sciences
University of Malaga
29071-Málaga, Spain
Tel: 34952131862
Fax: 34952136645
E-mail: [email protected]

Received Date: June 30, 2011; Accepted Date: November 05, 2011; Published Date: November 15, 2011

Citation: Tapia-Paniagua ST, Reyes-Becerril M, Ascencio-Valle F, Esteban MÁ, Clavijo E, et al. (2011) Modulation of the Intestinal Microbiota and Immune System of Farmed Sparus aurata by the Administration of the Yeast Debaryomyces hansenii L2 in Conjunction with Inulin. J Aquac Res Development S1:012. doi:10.4172/2155-9546.S1-012

Copyright: © 2011 Tapia-Paniagua ST, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Fish gastrointestinal tract is one of the most important sites of interaction with the external world. Interactions between the intestinal microbiota and the host modulate the functionality of the intestinal mucosa and gene expression. Application of probiotics, prebiotics and synbiotics for aquatic animals is increasing to improve welfare and promote growth. However, data about the use of probiotics in conjunction with prebiotics, in farmed marine organisms are scarce. The objective of this work is to study the modulation ability of the intestinal microbiota and the immune system of gilthead seabream exerted by the probiotic yeast Debaromycess hansonii L2 in conjunction with the prebiotic inulin. Fish were fed a commercial diet (control diet I), and with the same diet supplemented with 1.1% D. hansenii strain L2 (106 CFU g-1) plus inulin (3%) (diet II) for 4 weeks. The whole intestines of healthy fish from each group were aseptically removed at 2 and 4 weeks after starting the experiment and they were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE). Samples of blood and head kidney were obtained for humoral and cellular immune parameters determination. The expression of 12 selected genes related to the immune response (IgM, MHCI?, MHCII?, C3, IL-1?,TLR, TNF?, CSF-1R, NCCRP-1, Hep, TCR? y CD8) were analyzed by real-time PCR from skin, intestine, liver and head-kidney tissue. In this study, relevant changes in the intestinal microbiota of gilthead seabream specimens fed the diets assayed has been demonstrated. An important effect on the intestinal microbiota by the dietary administration of a synbiotic mixture has also been detected, especially in fish receiving the supplemented diet for 4 weeks. These changes coincided in the same time with an up-regulation of the expression of immunological genes in skin and head kidney of gilthead seabream at 2 and 4 weeks, respectively.

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