alexa Molar Concentration Welcomes Avogadro in Postgenomic Analytics | OMICS International | Abstract
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
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Research Article

Molar Concentration Welcomes Avogadro in Postgenomic Analytics

Andrey V Lisitsa*, Elena A Ponomarenko, Olga I Kiseleva, Ekaterina V Poverennaya and Alexander I Archakov

Institute of Biomedical Chemistry, 119121, Pogodinskaya Street 10, Moscow, Russian Federation

*Corresponding Author:
Andrey V Lisitsa
Institute of Biomedical Chemistry, 119121
Pogodinskaya Street 10, Moscow, Russian Federation
Tel: (+7) 499 2463731
E-mail: [email protected]

Received Date: October 30, 2015; Accepted Date: November 05, 2015; Published Date: November 07, 2015

Citation: Lisitsa AV, Ponomarenko EA, Kiseleva OI, Poverennaya EV, Archakov AI (2015) Molar Concentration Welcomes Avogadro in Postgenomic Analytics. Biochem Anal Biochem 4:216. doi:10.4172/2161-1009.1000216

Copyright: © 2015 Lisitsa AV et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The researchers working with high-throughput methods of genomics, transcriptomics, and proteomics reconsider the concept of concentration and evaluate the data obtained in the number of copies of biomacromolecules. Measurement of copy number reflects a steady trend in increasing the sensitivity of postgenomic analytical methods, up to the level of a single molecule. In this paper we review the physical meaning of the terms "molar concentration" and "Avogadro’s number" to establish a relationship between them. The relationship between the molar concentration and the number of copies of that same macromolecule in a certain volume is set through the reverse Avogadro’s number, the value of which (10-24 М) characterizes the molar concentration of a single molecule in 1 liter. Using the reverse Avogadro’s number, we deal with situations in analyzing homogeneous biological solutions and heterogeneous cellular material.

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