alexa Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C | OMICS International | Abstract
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
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Research Article

Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C

Alexandra Poturnayova1,2, Michael Leitner3, Maja Snejdarkova2, Peter Hinterdorfer4, Tibor Hianik1 and Andreas Ebner4*

1Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynska dolina, F1 Bratislava, 84248, Slovakia

2Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28 Ivanka pri Dunaji, Slovakia

3Center for Advanced Bioanalysis GmbH, Gruberstrasse 40, 4020 Linz, Austria

4Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria

*Corresponding Author:
Andreas Ebner
Institute of Biophysics, Johannes Kepler University Linz
Gruberstrasse 40, 4020 Linz, Austria
Tel: +43 732 2468 7637
Fax: +43 732 2468 7633
E-mail: [email protected]

Received Date: April 02, 2014; Accepted Date: May 15, 2014; Published Date: May 20, 2014

Citation: Poturnayova A, Leitner M, Snejdarkova M, Hinterdorfer P, Hianik T, et al. (2014) Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C. J Nanomed Nanotechnol 5:202. doi:10.4172/2157-7439.1000202

Copyright: © 2014 Poturnayova A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

By means of the atomic force microscopy techniques we studied the surface topography of the supported bilayer lipid membranes (sBLM) composed of 1,2-sn-glycerodimyristoylfosfatidylcholine (DMPC) with incorporated calixarenes tOct [6]CH2COOH (CX) specific to cytochrome c (cyt c). It is supposed that cyt c interacts with CX through amino groups of lysine residues at its surface. Therefore we also applied single molecule force spectroscopy (SMFS) to analyze the mechanisms of interaction of cyt c with the CX. In later case cyt c or individual NH2 group have been connected to the AFM tip through special linker. The topography of bare sBLM in a gel state (T=19ºC) revealed relatively smooth surface (RRMS=0.18 nm) and thickness ~5.1 nm which agrees well with previous studies. Incorporation of CX into DMPC bilayer resulted in increase of the surface roughness (RRMS= 0.39 nm) and in increase of thickness in average by 0.5 nm. The incubation of the layer with30 nM of cyt c resulted in a surface smoothing (RRMS=0.32 nm) and in a further increase of the thickness between 0.7 to 1.2 nm. The SMFS experiments with cyt c modified AFM tips approved its specific binding to CX and allowed us to determine the binding parameters koff (1.14 ± 0.59 s-1) and xβ (3.98 ± 0.63Å). SMFS experiments with an amino-ended linker also resulted in highly specific interactions with comparable values for koff (2.74 ± 0.66 s-1) and xβ (5.91 ± 2.55 Å). This suggests that both electrostatic and amino group specific interactions between cyt c and CX cavity exist.

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