alexa Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C

Alexandra Poturnayova1,2, Michael Leitner3, Maja Snejdarkova2, Peter Hinterdorfer4, Tibor Hianik1 and Andreas Ebner4*

1Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynska dolina, F1 Bratislava, 84248, Slovakia

2Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28 Ivanka pri Dunaji, Slovakia

3Center for Advanced Bioanalysis GmbH, Gruberstrasse 40, 4020 Linz, Austria

4Institute of Biophysics, Johannes Kepler University Linz, Gruberstrasse 40, 4020 Linz, Austria

*Corresponding Author:
Andreas Ebner
Institute of Biophysics, Johannes Kepler University Linz
Gruberstrasse 40, 4020 Linz, Austria
Tel: +43 732 2468 7637
Fax: +43 732 2468 7633
E-mail: [email protected]

Received Date: April 02, 2014; Accepted Date: May 15, 2014; Published Date: May 20, 2014

Citation: Poturnayova A, Leitner M, Snejdarkova M, Hinterdorfer P, Hianik T, et al. (2014) Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C. J Nanomed Nanotechnol 5:202. doi:10.4172/2157-7439.1000202

Copyright: © 2014 Poturnayova A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

By means of the atomic force microscopy techniques we studied the surface topography of the supported bilayer lipid membranes (sBLM) composed of 1,2-sn-glycerodimyristoylfosfatidylcholine (DMPC) with incorporated calixarenes tOct [6]CH2COOH (CX) specific to cytochrome c (cyt c). It is supposed that cyt c interacts with CX through amino groups of lysine residues at its surface. Therefore we also applied single molecule force spectroscopy (SMFS) to analyze the mechanisms of interaction of cyt c with the CX. In later case cyt c or individual NH2 group have been connected to the AFM tip through special linker. The topography of bare sBLM in a gel state (T=19ºC) revealed relatively smooth surface (RRMS=0.18 nm) and thickness ~5.1 nm which agrees well with previous studies. Incorporation of CX into DMPC bilayer resulted in increase of the surface roughness (RRMS= 0.39 nm) and in increase of thickness in average by 0.5 nm. The incubation of the layer with30 nM of cyt c resulted in a surface smoothing (RRMS=0.32 nm) and in a further increase of the thickness between 0.7 to 1.2 nm. The SMFS experiments with cyt c modified AFM tips approved its specific binding to CX and allowed us to determine the binding parameters koff (1.14 ± 0.59 s-1) and xβ (3.98 ± 0.63Å). SMFS experiments with an amino-ended linker also resulted in highly specific interactions with comparable values for koff (2.74 ± 0.66 s-1) and xβ (5.91 ± 2.55 Å). This suggests that both electrostatic and amino group specific interactions between cyt c and CX cavity exist.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords