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ISSN: 2168-9547

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Research Article

Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells

Chika Nakagawa1 Kaori Senda-Murata2, Ai Kawakita-Yamaguchi2, Takashi Fukada1, and Kenji Sugimoto1,2*

1Laboratory of Applied Molecular Biology, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, 599-8531 Japan

2Live Cell Imaging Institute, Osaka Prefecture University, Osaka, 599-8531 Japan

*Corresponding Author:
Kenji Sugimoto
Live Cell Imaging Institute, Osaka Prefecture University
Osaka, 599-8531 Japan
E-mail: [email protected]

Received date: March 26, 2016 Accepted date: March 11, 2016 Published date: March 18, 2016

Citation: Nakagawa C, Senda-Murata K, Kawakita-Yamaguchi A, Fukada T, Sugimoto K (2016) Molecular Behavior of CENP-A and PCNA throughout the Cell Cycle in Living Human HT-1080 Cells. Mol Biol 5:160. doi: 10.4172/2168-9547.1000160

Copyright: © 2016 Nakagawa C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Centromere is the chromosomal region to which spindle microtubules attach in mitosis. Centromere protein A (CENP-A), a centromere-specific histone H3 variant [1], was originally identified by anti-centromere autoantibodies [2]. Human CENP-A is assembled into prekinetochores in G1 phase, and is stably maintained through multiple cell divisions [3]. We previously reported the relative position of three centromere auto antigens, CENP-A, CENP-B, and CENP-C, in human MDA435 cells in interphase nuclei and metaphase chromosomes [4]. We then characterized the molecular behavior of CENP-A and heterochromatin protein 1α (HP1α) during mitosis in living MDA435 cells [5]. Recently, Lidsky et al. reported the dynamics of Cid/CENP-A, CENP-C, and Cal 1 in Drosophila S2R+ cells, and presented the live-cell images stably expressing the Cid-EGFP and mCherry-PCNA through the cell cycle [6]. However, the localization of mammalian CENP-A throughout the cell cycle in living cells is unclear.

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