alexa Molecular Breeding of Solasodine Glycoside for Solanum khasianum using Anti-Solamargine Single-Chain Fv Gene
ISSN: 2167-0412

Medicinal & Aromatic Plants
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Review Article

Molecular Breeding of Solasodine Glycoside for Solanum khasianum using Anti-Solamargine Single-Chain Fv Gene

Waraporn Putalun1, Hiroyuki Tanaka2, Seiichi Sakamoto3 and Yukihiro Shoyama3*
1Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
2Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Japan
3Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki, Japan
Corresponding Author : Yukihiro Shoyama
Faculty of Pharmaceutical Sciences
Nagasaki International University, 2825-7 Huis Ten Bosch
Sasebo, Nagasaki 859-3298, Japan
E-mail: [email protected], [email protected]
Received March 05, 2013; Accepted March 20, 2013; Published March 22, 2013
Citation: Putalun W, Tanaka H, Sakamoto S, Shoyama Y (2013) Molecular Breeding of Solasodine Glycoside for Solanum khasianum using Anti-Solamargine Single-Chain Fv Gene. Med Aromat Plants 2:124. doi:10.4172/2167-0412.1000124
Copyright: © 2013 Putalun W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A recombinant single chain fragment variable (scFv) antibody was constructed from hybridoma cell lines expressing anti-solamargine (As) monoclonal antibody (MAb). The characteristics of the As-scFv protein, expressed in both recombinant Escherichia coli and hairy root, were almost same with those of the original MAb. Up to 220 ng recombinant As-scFv was produced per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The solasodine glycosides concentration was 2.3 fold higher in the transgenic, than in the wild-type hairy root, reflecting from the soluble As-scFv level. The recombinant S. khasianum plant regenerated from recombinant hairy roots contained 2.3 fold concentration of solasodine glycoside, compared to the regenerated plant from wild-type hairy root. These results suggested that the scFv antibody expressed in transgenic plant controlled the antigen level. This methodology is the first success for molecular breeding of secondary metabolites, without understanding of biosynthetic enzyme.

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